文献类型：期刊文献

英文题名：Identification of aldehyde oxidase 3 as a binding protein for squid ink polysaccharides using magnetic nanoparticles

作者：Lin, Zhen[1];Tan, Xiaohui[1];Li, Fangping[1];Zhang, Yu[1];Luo, Ping[1];Lin, Xuan[1];Liu, Huazhong[1]

机构：[1]Guangdong Ocean Univ, Coll Chem & Environm Sci, Zhanjiang 524088, Peoples R China

年份：2021

卷号：11

期号：6

起止页码：3596

外文期刊名：RSC ADVANCES

收录：SCI-EXPANDED(收录号：WOS:000611287900043)、、EI(收录号：20210509853792)、Scopus(收录号：2-s2.0-85100044326)、WOS

基金：This work was supported by the Natural Science Foundation of Guangdong Province, China (2019A1515011102), the Science and Technology Project on Special Fund for Public Welfare Research and Ability Construction of Guangdong Province, China (2017A010105010), and the Project of Application-Based Talent Training Course from Guangdong Ocean University (570319017).

语种：英文

外文关键词：Nanomagnetics - Nanoparticles - Proteins - Shellfish - Mammals - Molluscs - Aldehydes

外文摘要：To explore the interactive molecules of squid ink polysaccharides (SIP) for further understanding the action mechanisms of SIP bio-function, this study prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads. Michaelis-Menten constant (K-m) was detected from a Lineweaver-Burk double reciprocal plot to assess effect of SIP on activity of aldehyde oxidase (AOX). Results showed that three proteins, AOX-3, regucalcin (RGN) and alpha 1-antitrypsin (A1AT3) were separated from mouse liver by magnetic nanoparticles conjugated with SIP. Contents of AOX-3 were much more than RGN and A1AT3. SIP (0.5 mg mL(-1)) reduced K-m value of aldehyde oxidase of mouse liver from 91.79 mu mol L-1 to 43.70 mu mol L-1.