文献类型：期刊文献

英文题名：Effect of carboxymethylation on properties of fucoidan from Laminaria japonica: Antioxidant activity and preservative effect on strawberry during cold storage

作者：Duan, Zhenhua[1];Duan, Weiwen[1];Li, Fangping[2];Li, Yanqun[3];Luo, Ping[2];Liu, Huazhong[2]

机构：[1]Hezhou Univ, Inst Food Sci & Engn Technol, Hezhou 542899, Peoples R China;[2]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

年份：2019

卷号：151

起止页码：127

外文期刊名：POSTHARVEST BIOLOGY AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000459827400015)、、WOS

基金：This work was jointly supported by the Special Fund for Distinguished Experts in the Guangxi Zhuang Autonomous Region, Guangxi Talent Highland of Preservation and Deep Processing Research in Fruit and Vegetables, Special Fund for Science and Technology Base and Talent of Guangxi (GKAD17195088).

语种：英文

外文关键词：Carboxymethylation; Fucoidan; Laminaria japonica; Antioxidant activity; Strawberry fruit

外文摘要：Carboxymethylation modification is commonly used for improving physicochemical and biological properties of nature polysaccharides, effect of carboxymethylation on property of fucoidan from Laminaria japonica (FL) is still unknown even though the polysaccharide has been isolated for several decades. To explore the derivatization-related effect, this study prepared carboxymethyl FL (CMFL) using chloroacetic acid. CMFL was characterized by degree of substitution (DS) determination and infrared spectroscopic analysis. Antioxidant activity of CMFL in vitro was assessed by scavenging capacity of DPPH, hydroxyl and superoxide radicals. Strawberry were used to investigate the effects of CMFL on harvested fruit. DS was determined to be 0.6733, and two characteristic absorption peaks of carboxymethylation were detected at 1602 and 1420 cm(-1), suggesting that FL was carboxymethylated. IC50 values of hydroxyl, superoxide and DPPH radicals decreased by 17.8%, 17.5% and 15.7%, respectively, indicating higher in vitro antioxidant activity of CMFL than FL. CMFL reduced water loss and decay more effectively than FL. CMFL and to a lesser extent, FL treated fruit had higher ascorbic acid content and stronger scavenging capacities of DPPH, hydroxyl and superoxide radicals than control fruit during storage. Overall, carboxymethylation can enhance the properties of FL, including its effects on antioxidant activity, which suggests that CMFL can be used as a natural preservative for harvested fruit.