文献类型：期刊文献

中文题名：降胆固醇牡蛎肽的构效关系解析: 肽组学与生物信息学整合研究

英文题名：Deciphering the Structure-Activity Relationships of Cholesterol-Lowering Oyster Peptides: an Integrated Peptidomics and Bioinformatics Study

作者：Zhang, Hongyu[1]; Wang, Yu’Ou[1]; Chen, Zhongqin[1,2,3,4]; Tan, Mingtang[1,2,3,4]; Chen, Ming[1,2,3,4]; Gao, Jialong[1,2,3,4]; Zheng, Huina[1,2,3,4]; Lin, Haisheng[1,2,3,4]; Cao, Wenhong[1,2,3,4]

机构：[1] College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 524088, China; [2] National Research and Development Branch Center for Shellfish Processing [Zhanjiang], Zhanjiang, 524088, China; [3] Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang, 524088, China; [4] Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Zhanjiang, 524088, China

年份：2026

卷号：47

期号：11

起止页码：78

外文期刊名：Shipin Kexue/Food Science

收录：EI(收录号：20263421346735)

语种：中文

外文关键词：Cholesterol - Chromatographic analysis - Drug products - Enzyme inhibition - Ion chromatography - Ion exchange - Mass spectrometry - Medicinal chemistry - Molecular docking - Peptides

外文摘要：This study utilized peptidomics and bioinformatics to investigate the structure-hypocholesterolemic activity relationships of oyster peptides (OPs). Ion-exchange chromatography separated OP into three fractions (OP-1, OP-2, and OP-3). OP-2 showed the strongest inhibitory effect on pancreatic lipase (PL) (76.16%) and cholesterol esterase (CE) (66.43%), reducing total cholesterol (TC) in a concentration-dependent manner. Analysis by liquid chromatography-mass spectrometry (LC-MS) indicated that active peptides (5 cells/well in six-well plates) by reducing intracellular cholesterol accumulation and enhancing low-density lipoprotein (LDL) uptake. At 50 μmol/L, its cholesterol lowering effect was similar to that of simvastatin at 10 μmol/L. These findings highlight the structural motifs crucial for dual-enzyme inhibition and hepatic cholesterol regulation, emphasizing the potential of OPs as a natural cholesterol-lowering agent and promising candidate for future therapeutic development. Further studies on their gastrointestinal stability and in vivo efficacy are warranted to fully assess their potential for dietary intervention. ? 2026, Chinese Chamber of Commerce. All rights reserved.