文献类型：期刊文献

英文题名：Cadmium-chelating ability of the siderophore DHBS secreted by Leclercia adecarboxylata FCH-CR2 and its action mechanism

作者：Guan, Wenhao[1]; Fang, Zhijia[1]; Chen, Yinyan[1]; Li, Yongbin[1]; Peng, Zhilan[2]; Sun, Lijun[1]; Deng, Qi[1]; Gooneratne, Ravi[3]

机构：[1] College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, China; [2] The Marine Biomedical Research Institute, Guangdong Medical University, Zhanjiang, 524023, China; [3] Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, Canterbury, 7647, New Zealand

年份：2023

卷号：900

外文期刊名：Science of the Total Environment

收录：EI(收录号：20233114484437)、Scopus(收录号：2-s2.0-85166290129)

语种：英文

外文关键词：Amino acids - Cadmium - Health risks - RNA - Soil pollution

外文摘要：As one of the most accumulative toxic heavy metals, cadmium (Cd) poses a major threat to human health. Bacterial siderophores, as small molecules with metal-absorbing ability, have great potential activity for Cd-reduction. In this study, the siderophore-producing bacterialstrain FCH-CR2 was isolated from a high-Cd contaminated soil using the CAS method. Leclercia adecarboxylata was identified through 16S rRNA sequence, homology analysis, colony morphology, physiological and biochemical tests. A siderophore, catechol type 2,3-dihydroxy-N-benzoyl-L-serine (DHBS) secreted by FCH-CR2, was purified using RP-HPLC and identified by LC-MS/MS. Intraperitoneal injection of DHBS significantly increased fecal Cd levels, and reduced Cd accumulation in organs. In density flooding theory (DFT) analysis, DHBS may bind to Cd via the hydroxyl site on the benzene ring. Besides, the isothermal titration calorimetry (ITC) assay revealed that the formation of Cd-DHBS is a spontaneous and endothermic reaction with ΔG = ?21.4 kJ/mol and ΔH = 1.51 ± 0.142 kJ/mol. ? 2023 Elsevier B.V.