文献类型：期刊文献

英文题名：Enhanced Biomass and Astaxanthin Production ofHaematococcus pluvialisby a Cell Transformation Strategy with Optimized Initial Biomass Density

作者：Li, Feng[1,2,3,4];Cai, Minggang[1,2,3,4];Lin, Mingwei[4];Huang, Xianghu[1];Wang, Jun[4,5];Ke, Hongwei[3,4];Wang, Chunhui[3,4];Zheng, Xuehong[3,4];Chen, Ding[3,4];Yang, Shihui[4]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Xiamen Univ, Fujian Prov Key Lab Coastal Ecol & Environm Studi, Xiamen 361102, Peoples R China;[3]Key Lab Marine Chem & Appl Technol, Xiamen 361101, Peoples R China;[4]Xiamen Univ, Coll Ocean & Earth Sci, Xiamen 361101, Peoples R China;[5]Xiamen Ocean Vocat Coll, Dept Biotechnol, Xiamen 361101, Peoples R China

年份：2020

卷号：18

期号：7

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000557117000001)、、WOS

基金：This research was mainly supported by Xiamen Southern Ocean Technology Center of China (grant number 14CZP035HJ09) and partly funded by Xiamen Scientific and Technologic Projects (grant number 3052Z20031086 and 3052Z20123004), Marine Science Base Scientific Research Training and Scientific Research Ability Enhancement Project of Xiamen University (grant number J1210050), Xiamen University Training Program of Innovation and Entrepreneurship for Undergraduates (grant number 2016X0619), Development and application of micro-plastic detection technology for coastal environment in Xiamen (XTZX-HYSW-1802), the Special Funds for Scientific Research of Marine PublicWelfare Industry (grand number 201305016), and the funding of Fujian Marine Biological Application Technology Collaborative Innovation Center, Fujian Ocean Vocational School (No. WTZX-HYSW-1802).

语种：英文

外文关键词：Haematococcus pluvialis; astaxanthin; encystment; cell transformation; initial biomass density

外文摘要：Astaxanthin fromH. pluvialisis an antioxidant and presents a promising application in medicine for human health. The two-stage strategy has been widely adopted to produce astaxanthin by theHaematococcusindustry and research community. However, cell death and low astaxanthin productivity have seriously affected the stability of astaxanthin production. This study aims to test the effect of cell transformation strategies on the production of astaxanthin fromH. pluvialisand determine the optimal initial biomass density (IBD) in the red stage. The experimental design is divided into two parts, one is the vegetative growth experiment and the other is the stress experiment. The results indicated that: (1) the cell transformation strategy ofH. pluvialiscan effectively reduce cell death occurred in the red stage and significantly increase the biomass and astaxanthin production. (2) Compared with the control group, the cell mortality rate of the red stage in the treatment group was reduced by up to 81.6%, and the biomass and astaxanthin production was increased by 1.63 times and 2.1 times, respectively. (3) The optimal IBD was determined to be 0.5, and the highest astaxanthin content can reach 38.02 +/- 2.40 mg center dot g(-1). Thus, this work sought to give useful information that will lead to an improved understanding of the cost-effective method of cultivation ofH. pluvialisfor natural astaxanthin. This will be profitable for algal and medicine industry players.