文献类型：期刊文献

英文题名：Effects of Chinese herbal medicines on growth performance, intestinal flora, immunity and serum metabolites of hybrid grouper (Epinephelus fuscoguttatus ♀ x Epinephelus lanceolatu ♂)

作者：Cai, Jia[1,2];Yang, Zhenggao[1];Huang, Yu[1];Jian, Jichang[1,3];Tang, Jufen[1,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China;[2]Guangxi Acad Sci, Guangxi Key Lab Aquat Biotechnol & Modern Ecol Aqu, Nanning 530007, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Fisheries Coll, 1 Haida Rd,Mazhang Dist, Zhanjiang 524088, Guangdong, Peoples R China

年份：2023

卷号：140

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001043632800001)、、WOS

基金：Acknowledgments The authors thank OE Biotech Co., Ltd. (Shanghai, China) and Xiaoxin Ye for their assistant for the analysis of intestinal microbiota and serum metabolites. This work was supported by National Natural Sci-ence Foundation of China (U20A2065, 32073006, 32002426) , National Key R & D Program of China (2018YFD0900501) , Natural Science Foundation of Guangdong Province (2021B0202040002) and Natural Science Foundation of Guangxi (2020GXNSFAA297243) .

语种：英文

外文关键词：female Epinephelus fuscoguttatusx male Epinephelus; lanceolatu; CHM; Growth performance; Intestinal microbiota; Immunity; Serum metabolism

外文摘要：The immunomodulatory roles of Chinese herbal Medicine (CHM) in aquatic animals have been well-recorded. However, how CHM impacts the intestinal microbiota and serum metabolism is not fully understood. In this study, the effects of different additive levels of CHM on the growth performance, immunity, intestinal flora and serum metabolism of hybrid grouper (female Epinephelus fuscoguttatus x male Epinephelus lanceolatu) were investigated. The addition of 0.5%, 1.0%, 1.5% and 2.0% Chinese herbal medicine compound to feed could significantly improve the weight gain rate (WGR), specific growth rate (SGR) and survival rate (SR) of grouper, reduced feed coefficient, while had no significant difference on morphometric parameter. The most significant improvement for the parameters above was observed in 1.5% group. Different addition levels of CHM could also significantly enhance the activities of ACP, AKP, SOD, CAT and LZM in serum. Accordingly, the supplementation of CHM significantly induced up-regulation of immune genes such as IL-8, IL-1 beta, TNF-alpha, Nrf2, Lzm in the liver, spleen and head kidney of grouper, improved the resistance of grouper to V. harveyi as well. The intestinal flora analysis showed that at the phylum level, the main dominant species of intestinal microorganisms were Firmicutes, Proteobacteria, Bacteroidota, Actinobacteriota, Gemmatimonadota, Desulfobacterota, Fusobacteriota and Myxococcota. At the genus level, the high abundance was Lactobacillus, Streptococcus, Bacteroides, Escherichia, Romboutsia, Sphingomonas and Muribaculaceae. The abundance of probiotics (such as Lachnospiraceae, Lactobacillaceae, Streptococcaceae, etc) in CHM-supplement groups were higher (highest in 1.5% group) compared with control group. Moreover, a total of 11 common differential metabolic pathways were screened by LC-MS metabolism analysis of serum, they were Neuroactive ligand-receptor interaction, Purine metabolism, Linoleic acid, Glycerophospholipid metabolism, Taurine and hypotaurine metabolism, Arginine and proline metabolism, ABC transporters, Aminoacyl-tRNA biosynthesis, Arachidonic acid metabolism, Drug metabolismcytochrome P450, alpha-Linolenic acid metabolism. Also, three common differential metabolites (PI(20:4 (5Z,8Z,11Z,14Z)/18:1(11Z)), PC(20:3(8Z,11Z,14Z)/22:1(13Z)), PC(22:0/20:4(5Z,8Z,11Z,14Z)) associated with intestinal health, growth and disease resistance was found. These data will contributes to a comprehensive understand for the regulatory roles of CHM on fish, which is also beneficial for the disease control and sustainable development of aquaculture.