文献类型：期刊文献

英文题名：Nitrogen removal characteristics and auto-aggregation capacity of the heterotrophic nitrification-aerobic denitrification bacterium, Bacillus amyloliquefaciens N8

作者：Liu, Bo[1,2];Liu, Xingyuan[1,2];Zhang, Wenqian[1,2];Wang, Tingyu[1,2];Tan, Beiping[3];Ye, Chaoxia[1,2]

机构：[1]South China Normal Univ, Inst Insect Sci & Technol, Guangzhou Key Lab Subtrop Biodivers & Biomonitorin, Guangdong Prov Key Lab Insect Dev Biol & Appl Tech, Guangzhou 510631, Peoples R China;[2]South China Normal Univ, Sch Life Sci, Guangzhou 510631, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Nutr & Feed, Zhanjiang, Peoples R China

年份：2025

卷号：157

起止页码：256

外文期刊名：PROCESS BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:001551914200001)、、EI(收录号：20253018846211)、Scopus(收录号：2-s2.0-105011366057)、WOS

基金：This research was supported by National Key R & D Program of China (grant number 2023YFD2402000) , Guangzhou Municipal Science and Technology Project (grant number 2023E04J1247) and Guangdong MEPP Fund (grant number GDOE [2019] A33) .

语种：英文

外文关键词：Bacillus amyloliquefaciens; Heterotrophic nitrification; Aerobic denitrification; Nitrogen removal pathway; Auto-aggregation capacity

外文摘要：At present, the main factors restricting the development of high-salinity recirculating aquaculture systems are high costs and the control of inorganic nitrogen pollutants, but only a few studies have reported on salt-tolerant HN-AD strains and their denitrification mechanisms and quantification capabilities. Strain N8 was obtained from seawater biofilter and identified as Bacillus amyloliquefaciens based on the analysis of 16S rRNA gene sequence. Strain N8 has a good ability to remove ammonia nitrogen, nitrite and nitrate at three different concentrations (20, 50, 100 mg L- 1) with little accumulation of intermediates, and the maximum removal rate of ammonia nitrogen (50 mg L- 1), nitrite (100 mg L- 1), and nitrate (100 mg L- 1) were 96.56 %, 99.20 % and 90.20 % after 72 h incubation. Through inhibitor and nitrogen balance experiments, it was found that the first step of degradation of ammonia nitrogen by strain N8 was to oxidize it to hydroxylamine, and 72.28 % of ammonia nitrogen and 46.31 % of nitrate nitrogen were eventually converted into intracellular nitrogen. Two denitrification related genes, nirK and nirS, were successfully amplified from strain N8. The ideal conditions for growth and nitrogen removal of strain N8 were sodium succinate as the carbon source, C/N of 20, and salinity between 0 and 30 parts per thousand. Furthermore, the highest auto-aggregation rate of the strain was above 25 % under different nitrogen sources, and the biofilm growth amount of the strain was 3.04 after 24 h culture. All results demonstrate that strain N8 has great practical application potential in nitrogen removal in high-salinity recirculating aquaculture systems.