文献类型：期刊文献

中文题名：弓首蛔虫及狮弓蛔虫线粒体基因组nad4基因多态性研究

英文题名：Analysis of DNA Polymorphism in Mitochondrial NADH Dehydrogenase Subunit 4 in Toxocara Species and Toxascaris leonina

作者：李明伟[1];林瑞庆[2];曹湛[2];魏冬霞[2];邹丰才[2];宋慧群[2];朱兴全[2]

机构：[1]广东海洋大学农学院动物医学系;[2]华南农业大学兽医学院寄生虫学研究室

年份：2006

卷号：6

期号：3

起止页码：236

中文期刊名：热带医学杂志

外文期刊名：Journal of Tropical Medicine

收录：CSTPCD

基金：国家杰出青年科学基金(No.30225033);教育部"留学回国人员科研启动基金"(教外司留[2003]406号);教育部"高等学校博士学科点专项科研基金"(No.20040564008)。

语种：中文

中文关键词：犬弓首蛔虫;猫弓首蛔虫;马来西亚弓首蛔虫;牛弓首蛔虫;狮弓蛔虫;线粒体DNA;烟酰胺脱氢酶亚基Ⅳ基因;PCR-SSCP

外文关键词：Toxocara canis ; Toxocara cati; Toxocara malaysiensis ; Toxocara vitulorum ; Toxascaris leonina; mitochondrial DNA ; had4; PCR-SSCP

中文摘要：目的比较犬、猫常见蛔虫的线粒体DNA(mtDNA)中烟酰胺脱氢酶亚基Ⅳ基因(nad4)部分序列(pnad4),以期找出它们之间的序列差异和种群遗传关系。方法抽提61个弓首属蛔虫(包括犬弓首蛔虫、猫弓首蛔虫、马来西亚弓首蛔虫和犊弓首蛔虫)和狮弓蛔虫虫体的总DNA,用PCR方法扩增mtDNApnad4,然后用SSCP技术和DNA测序对序列进行分析研究。结果犬弓首蛔虫种群内pnad4的序列差异为1.17%,与猫弓首蛔虫、马来西亚弓首蛔虫、牛弓首蛔虫和狮弓蛔虫种间的平均序列差异分别为13.92%、16.48%、15.12%和20.32%。猫弓首蛔虫种群内pnad4的序列差异为1.00%,与马来西亚弓首蛔虫、牛弓首蛔虫、狮弓蛔虫平均序列差异分别是17.90%、13.55%和18.50%。马来西亚弓首蛔虫种群内pnad4序列差异为0.13%,与牛弓首蛔虫、狮弓蛔虫序列差异分别为13.50%和20.60%。结论犬弓首蛔虫不同地方虫株的pnad4有一定差异,猫弓首蛔虫种群内差异较小,马来西亚弓首蛔虫种群内差异很小。弓首属各虫种之间的差异以及与狮弓蛔虫的差异都较大。马来西亚弓首蛔虫pnad4序列与其它虫种差异都较大,证明它确为一独立种。pnad4序列是弓首蛔虫和狮弓蛔虫理想的种特异的遗传标记。

外文摘要：Objective To analyze the genetic relationship and the variation of the mitochondrial NADH dehydrogenase subunit 4 （nad4） genes in Toxocara species and Toxascaris leonina. Methods Genomic DNA from 61 samples of ascaridoid nematodes （ Toxocara canis , Toxocara cati, Toxocara malaysiensis , Toxocara vitulorum and Toxascwis leonina） were collected from different geographical locations. A portion of the mitochondrial NADH dehydrogenase subunit 4 gene （pnad4） from each sample was amplified by polymerase chain reaction （PCR）. Sequence heterogeneity in the pnad4 was examined and compared using single-strand conformation polymorphism （SSCP） analysis, followed by DNA sequencing analysis. Results The variation of pnad4 gene within the species of T. canis,T, carl, and T. malaysiensis was 1.17%, 1.00% and 0.13%, respectively. In the inter-species comparison, the differences of pnad4 gene were 13.92% between T.canis and T.cati, 16.48% between T.canis and T. rnalaysiensis, 15.12% between T. can/s and T. vitulorum, 20.32% between T. can/s and T. leonina, 17.90% between T. cati and T. malaysiensis, 13.55% between T. cati and T. vitulorum, 18.50% between T. cati and T. leonina, 13.50% between T. malaysiensis and T. v/tu/orum, and 20.60% between T. mahrys/ens/s and T. leonina. Conclusion The intraspecies variation of pnad4 was lower than the inter-species variation. The results also suggested that T.malayslensis is a distinct group of nematode, and pnad4 may be used as a genetic marker for the species identification. The results may also be used for the study of molecular systematics, population genetic structures and the molecular ecology of Toxocara and Toxacaris species.