文献类型：期刊文献

英文题名：Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility

作者：Li, P.[1,2];Zhou, L.[2,3];Wei, S.[2];Yang, M.[4];Ni, S.[2,3];Yu, Y.[2,3];Cai, J.[5];Qin, Q.[2,4]

机构：[1]Guangxi Acad Sci, Guangxi Key Lab Marine Nat Prod & Combinatorial B, Nanning 530007, Peoples R China;[2]Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[4]South China Agr Univ, Coll Marine Sci, Guangzhou, Guangdong, Peoples R China;[5]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China

年份：2017

卷号：90

期号：5

起止页码：1944

外文期刊名：JOURNAL OF FISH BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000400789500016)、、WOS

基金：This study was supported by the National Basic Research Programme of China (973) (Grant Number: 2012CB114402), the grant from the National Natural Science Foundation of China (Grant Number: 31330082) and Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences (LMB141005). We would like to thank J. Zhang from Scientific Equipment Service Centre of South China Sea Institute of Oceanology for flow cytometry technical assistance.

语种：英文

外文关键词：cytotoxicity; head-kidney cell line; Trachinotus ovatus; transfection efficiency; viral susceptibility

外文摘要：A cell line derived from the head kidney of golden pompano Trachinotus ovatus (TOHK) was established and characterized in this study. The TOHK cells grew most rapidly at 28 degrees C and the optimum foetal bovine serum concentration in L-15 medium was 10%. The TOHK cells have a diploid chromosome number of 2N = 54. The transfection efficiency of TOHK cells was 75% at the 15th passage and 72% at the 40th passage. The transfection efficiency in TOHK cells was high, so these cells are suitable for foreign gene expression. The cytotoxic effects of heavy metals and extracellular products from Vibrio anguillarum and Vibrio alginolyticus were demonstrated in TOHK cells, so this TOHK cell line could also be applied in environmental monitoring of heavy metals and pathogenic bacteria. TOHK cell line showed high virus susceptibility, such as grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). Then, TOHK cell line could be used for the study of viral pathogenesis and the development of antiviral strategies.