文献类型：期刊文献

中文题名：褪黑素抑制LPS诱导的脂肪细胞炎症反应及机制

英文题名：Effect and Mechanism of Melatonin on Inflammatory Response of 3T3-L1 Adipocyte Induced by LPS

作者：崔雁冰[1,2];陈绍红[1];刘铀[1];刘艳芬[2];赵云涛[1]

机构：[1]广东海洋大学食品科技学院现代生化中心;[2]广东海洋大学农学院,湛江524088

年份：2017

卷号：29

期号：2

起止页码：294

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD、、北大核心2014、CSCD2017_2018、北大核心、CSCD

基金：广东海洋大学自然科学基金(2007-1)

语种：中文

中文关键词：3T3-L1脂肪细胞;褪黑素;炎症反应;NF-κB

外文关键词：3T3-L1 adipocyte; melatonin; inflammatory response; NF-κB

中文摘要：研究褪黑素对脂多糖(Lipopolysaccharide,LPS)诱导的3T3-L1脂肪细胞炎症反应的抑制作用及机制。体外培养3T3-L1前脂肪细胞并诱导成为成熟的脂肪细胞,用不同浓度的褪黑素(50、100、200μmol/L)处理2 h后,加入LPS(2μg/m L)诱导炎症反应,于LPS处理后不同时间,取细胞,qRT-PCR测定肿瘤坏死因子α(Tumor Necrosis Factor Alpha,TNF-α)和白介素6(Interleukin-6,IL-6)的表达情况,Western blot法测定各组总蛋白p-IκBα的表达情况。取上清液,酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)检测TNF-α和IL-6的分泌情况。结果显示褪黑素显著抑制LPS诱导的3T3-L1脂肪细胞TNF-α和IL-6表达和分泌(P<0.05),呈现剂量效果依赖性;Western blot结果表明褪黑素显著抑制LPS诱导的脂肪细胞IκBα磷酸化(P<0.05)。这表明褪黑素通过降低LPS诱导的脂肪细胞IκBα磷酸化信号通路抑制脂肪细胞炎症反应。

外文摘要：This study aimed to explore the effect and mechanism of melatonin on inflammatory response of 3T3-L1 adipocyte induced by lipopolysaccharide（ LPS）. 3T3-L1 preadipocytes were cultured and induced to become mature adipose cells. Pretreated with different concentrations of melatonin for 2h,adipocytes were challenged with LPS（ 2 μg/m L）. After treatment with LPS for different time points,supernatant were harvested and enzyme linked immunosorbent assay（ ELISA） was used to detect the concentration of TNF-α and IL-6. qRT-PCRwere carried out to identify mRNA levels of TNF-α and IL-6. Western blot was used to assay p-IκBα. The results showed that 3T3-L1 adipocyte secreted TNF-α and IL-6 significantly after treatment with LPS. Melatoninsignificantly reduced TNF-α and IL-6 expression and secretion of3T3-L1 adipocyte induced by LPS in a dose-dependent manner. Western blot demonstrated that melatonin inhibited IκBαphosphorylation induced by LPS significantly. With these results,it was concluded that melatonin alleviated inflammatory response of 3T3-L1 adipocyte induced by LPS through inhibition of NF-κBsignal transduction.