文献类型：期刊文献

英文题名：PAX7promotes CD49f-positive dairy goat spermatogonial stem cells' self-renewal

作者：Du, Xiaomin[1];Wu, Siyu[1];Wei, Yudong[1];Yu, Xiuwei[1];Ma, Fanglin[1];Zhai, Yuanxin[1];Yang, Donghui[1];Zhang, Mengfei[1];Liu, Wenqing[1];Zhu, Haijing[1,2];Wu, Jiang[1,3];Liao, Mingzhi[4];Li, Na[1];Bai, Chunling[5];Li, Guangpeng[5];Hua, Jinlian[1]

机构：[1]Northwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China;[2]Yulin Univ, Life Sci Res Ctr, Yulin, Shaanxi, Peoples R China;[3]Guangdong Ocean Univ, Coll Agr, Zhanjiang, Peoples R China;[4]Northwest A&F Univ, Coll Life Sci, Yangling, Shaanxi, Peoples R China;[5]Inner Mongolia Univ, Key Lab Mammalian Reprod Biol & Biotechnol, Minist Educ, Hohhot, Peoples R China

年份：2021

卷号：236

期号：2

起止页码：1481

外文期刊名：JOURNAL OF CELLULAR PHYSIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000550477100001)、、Scopus(收录号：2-s2.0-85088272865)、WOS

基金：This study was supported by grants from the China National Basic Research Program (Grant No. 2016YFA0100203), the Program of National Natural Science Foundation of China (Grant No. 31572399), the Program of State Key Lab of Reproductive Regulation and Breeding of Grassland Livestock (Grant No. SKL-OT-201801), Science and Technology Major Project of Inner Mongolia Autonomous Region of China (Grant No. ZDZX2018065), and the Program of Shaanxi Province Science and Technology Innovation Team (Grant No. 2019TD-036) to Jinlian Hua.

语种：英文

外文关键词：CD49f; PAX7; self-renewal; spermatogonial stem cells

外文摘要：Spermatogenesis is a complex process that originates from and depends on the spermatogonial stem cells (SSCs). The number of SSCs is rare, which makes the separation and enrichment of SSCs difficult and inefficient. The transcription factor PAX7 maintains fertility in normal spermatogenesis in mice. However, for large animals, much less is known about the SSCs' self-renewal regulation, especially in dairy goats. We isolated and enriched the CD49f-positive and negative dairy goat testicular cells by magnetic-activated cell sorting strategies. The RNA- sequencing and experimental data revealed that cells with a high CD49f and PAX7 expression are undifferentiated spermatogonia in goat testis. Our findings indicated thatZBTB16 (PLZF),PAX7,LIN28A,BMPR1B,FGFR1, andFOXO1were expressed higher in CD49f-positive cells as compared to negative cells and goat fibroblasts cells. The expression and distribution of PAX7 in dairy goat also have been detected, which gradually decreased in testis tissue along with the increasing age. When the PAX7gene was overexpressed in dairy goat immortal mGSCs-I-SB germ cell lines, the expression of PLZF, GFR alpha 1, ID4, and OCT4 was upregulated. Together, our data demonstrated that there is a subset of spermatogonial stem cells with a high expression ofPAX7among the CD49f(+)spermatogonia, andPAX7can maintain the self-renewal of CD49f-positive SSCs.