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Sensitive fluorometric determination of platelet-derived growth factor BB and avian influenza A virus DNA via dual signal amplification using the hybridization chain reaction and glucose oxidase assisted recycling  ( SCI-EXPANDED收录 EI收录)   被引量:9

文献类型:期刊文献

英文题名:Sensitive fluorometric determination of platelet-derived growth factor BB and avian influenza A virus DNA via dual signal amplification using the hybridization chain reaction and glucose oxidase assisted recycling

作者:Li, Yubin[1];Shao, Jing[1];Guo, Wanting[1];Wang, Minting[1]

机构:[1]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China

年份:2019

卷号:186

期号:3

外文期刊名:MICROCHIMICA ACTA

收录:SCI-EXPANDED(收录号:WOS:000457555300028)、、EI(收录号:20242616409960)、Scopus(收录号:2-s2.0-85060946328)、WOS

基金:This work is supported by the Innovation Strong School Project of Guangdong education department (No. Q18291), the Non-funded Scientific and Technological Research Projects in Zhanjiang City (No. 2018B01005) and the program for scientific research start-up funds of Guangdong Ocean University (No. R17013).

语种:英文

外文关键词:Biomolecules detection; Silver coated glass slide; 3-(p-Hydroxyphenyl)-propanoic acid; Horseradish peroxidase; DNase I

外文摘要:A method is described for fluorometric determination of platelet-derived growth factor BB (PDGF-BB) and avian influenza A (H1N1) virus DNA. It is based on the use of the hybridization chain reaction (HCR) and of glucose oxidase (GOx) assisted dual-recycling amplification. A silver coated glass slide (SCGS) serves as an ideal material for separation. A signal DNA/initiator triggers the HCR and generates a cascade of hybridization to form a nicked double-helix polymer. Upon addition of the analytes (PDGF-BB or H1N1 DNA) and capture DNA immobilized on the SCGS, the nicked double-helix polymer binds on the surface of the SCGS through formation of a [capture DNA/analyte/signal DNA] sandwich structure. The GOx-biotin-streptavidin (SA) complexes were then attached to the nicked double-helix polymer through SA-biotin interaction. After cleavage by DNase I, the bound GOx is transferred into the buffer. Glucose is added and enzymatically oxidized to produce H2O2. The H2O2 formed oxidizes the substrate 3-(p-hydroxyphenyl)-propanoic acid to give a blue fluorescent product (with excitation/emission maxima at 320/416nm) under the catalysis of horseradish peroxidase. Under optimal conditions, fluorescence increases linearly in the 0.5 to 70pmolL(-1) PDGF-BB concentration range, and the detection limit is 191 fmolL(-1). For the H1N1 virus DNA, the respective data are 2.5 to 300pmolL(-1) and 826 fmolL(-1).

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