文献类型：期刊文献

中文题名：凡纳滨对虾腐败优势菌的分离鉴定及其群体感应信号分子测定

英文题名：Isolation and Identification of Dominant Spoilage Bacteria from Litopenaeus vannamei and Detection of Quorum Sensing Signaling Molecules

作者：李灿[1];张煜琛[1];孙力军[1];王雅玲[1];梁美晶[1];胡汉桥[1];徐德峰[1];刘颖[1];叶日英[1]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东湛江524088

年份：2016

卷号：37

期号：8

起止页码：164

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD、、北大核心2014、EI(收录号：20162402503503)、Scopus、北大核心、CSCD_E2015_2016、CSCD

基金：国家自然科学基金面上项目(31371746;31201309;31171634);“十二五”国家科技支撑计划项目(2012BAD29B06);广东省高等学校创新强校项目(GDOU2013050312;GDOU2013050205;GDOU2013050203)

语种：中文

中文关键词：凡纳滨对虾;优势腐败菌;群体感应;信号分子

外文关键词：Litopenaeus vannamei; dominant spoilage organism; quorurm sensing; signaling molecule

中文摘要：从凡纳滨对虾中分离和鉴定腐败优势菌,并对其进行群体感应信号分子AHLs和AI-2的测定。通过细菌自动生化鉴定系统和16S r DNA序列鉴定优势腐败菌;利用生物检测菌根癌农杆菌(Agrobacterium tumefaciens)JZA-1测定其AHLs、哈维弧菌(Vibrio harveyi)BB170检测其AI-2,并探究其动态生成规律。最终分离得到10株腐败优势菌,有4株菌均为变形杆菌属(Proteus),其他分别为Oceanisphaera profunda、溶血性葡萄球菌(Stapylococcus haemolyticus)、腐败希瓦氏菌(Shewanella putrefaciens)、短杆菌属(Brevibacterium)、Bacillus siamensis、Exiguobacterium aaestuarii。分离腐败优势菌中的6株革兰氏阴性菌均产生AHLs信号分子;变形杆菌属、腐败希瓦氏菌、短杆菌属、Bacillus siamensis产生AI-2信号分子。优势腐败菌的AHLs活性与菌体生长密度呈正相关,AI-2产生量在菌体生长对数期累积,在菌体生长24 h后活性迅速减弱。

外文摘要：This study was aimed at isolating,identifying the dominant spoilage bacteria from Litopenaeus vannamei using an automatic biochemical identification system and 16 S r DNA sequence,detecting the quorum sensing signaling molecules acyl homoserine lactones（AHLs）,autoinducer-2（AI-2）and exploring the dynamic formation regularities with indicator bacteria Agrobacterium tumefaciens JZA-1 and Vibrio harveyi BB170,respectively.Ten strains were selected and identified,including Oceanisphaera profunda,Stapylococcus haemolyticus,Shewanella putrefaciens,Brevibacterium sp.,Bacillus siamensis,Exiguobacterium aaestuarii and four strains of the Proteus genus.All the Gram-negative spoilage organisms isolated secreted signaling molecule AHLs while Proteus sp.,Shewanella putrefaciens,Brevibacterium sp.and Bacillus siamensis secreted signaling molecule AI-2.The AHLs activity of dominant spoilage organism was positively correlated with cell density.The AI-2 production was accumulated within the logarithmic phase and decreased rapidly from 24 h onwards.