文献类型：期刊文献

中文题名：基于COI基因和D-loop区部分序列比较雷州半岛东、西岸湖栖鳍虾虎鱼群体的遗传多样性

英文题名：Genetic diversity of Gobiopterus lacustris populations from western and eastern Leizhou Peninsula based on partial sequences of mitochondrial COI gene and D-loop

作者：廖健[1];龙水生[1];贺亮[1];郭昱嵩[1];刘楚吾[1];刘丽[1];王中铎[1]

机构：[1]广东海洋大学水产学院南海水产经济动物增养殖广东普通高校重点实验室,广东湛江524025

年份：2017

卷号：41

期号：2

起止页码：103

中文期刊名：海洋科学

外文期刊名：Marine Sciences

收录：CSTPCD、、北大核心2014、CSCD_E2017_2018、北大核心、CSCD

基金：国家自然科学基金项目(31201996);广东省高等学校优秀青年教师培养计划项目(Yq2013089);广东海洋大学优秀学位论文培育项目(201605)~~

语种：中文

中文关键词：COI;D-loop;湖栖鳍虾虎鱼(Gobiopterus;lacustris);雷州半岛群体;遗传分化

外文关键词：COI gene; D-loop region; Gobiopterus lacustris; population of Leizhou Peninsula; genetic differentiation

中文摘要：为比较分析雷州半岛东、西两岸湖栖鳍虾虎鱼(Gobiopterus lacustris)遗传差异,以COI基因和D-loop区部分序列为分子标记,获取雷州半岛湖栖鳍虾虎鱼群体COI序列44条(九龙山群体21条,高桥群体23条)、D-loop序列53条(九龙山群体25条,高桥群体28条)。基于COI基因部分序列分析结果显示:619 bp的44条序列中,统计的变异位点29个;T、C、A、G碱基组成分别为:27.8%、31.0%、23.7%、17.5%,且A+T(51.5%)高于C+G(48.5%);单倍型多样性比较,九龙山群体(0.695)>高桥群体(0.324);遗传分化分析表明东、西群体分化显著(Snn=0.0.591,0.01高桥群体(0.661);遗传分化分析表明东、西群体分化极显著(Snn=0.970,P<0.001),Fst为0.315(P<0.001),Nm值为0.55,Gst、Kxy、Dxy分别为:0.157、3.506、0.006;AMOVA分析揭示群体内变异程度高于群体间;聚类分析表明,基于COI基因与D-loop区部分序列构建的邻位链接法聚类树(NJ树)均存在按照采样点聚类的现象,但D-loop更为明显。综上,雷州半岛东、西两岸湖栖鳍虾虎鱼群体出现较为明显的分化现象。

外文摘要：We compared two Gobiopterus lacustris populations collected from the eastern and western Leizhou Peninsula based on COI gene and D-loop region.The partial sequences of COI gene from44individuals(21from eastern Jiulongshan population and23from western Gaoqiao population)and D-loop region from53individuals(25from JLS and28from GQ)were cloned.Based on COI gene,the results were as follows:there were29variable sites in619bp COI sequences.The frequencies of T,C,A,and G were27.8%,31.0%,23.7%,and17.5%,respectively,with more A+T(51.5%)than C+G(48.5%).The haplotype diversity of Jiulongshan population(0.695)was higher than that of Gaoqiao population(0.324).Genetic differentiation analysis indicated that the eastern and western populations were differentiated significantly(Snn=0.591,0.01