文献类型：期刊文献

英文题名：Identification and characterization of a novel bacteriocin PCM7-4 and its antimicrobial activity against Listeria monocytogenes

作者：Ma, Haotian[1];Ding, Yuexia[1];Peng, Jinju[1];Li, Yang[1];Pan, Ruixue[1];Long, Yuner[1];Zhao, Yining[1];Guo, Rongxian[2];Ma, Yi[1]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang 524088, Peoples R China;[2]Henan Univ Sci & Technol, Coll Anim Sci & Technol, Lab Funct Microbiol & Anim Hlth, Luoyang Key Lab Carrier Biomat & Anim Dis Prevent, Luoyang 471003, Peoples R China

年份：2025

卷号：290

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:001364744200001)、、EI(收录号：20244817424219)、Scopus(收录号：2-s2.0-85209888802)、WOS

基金：This work was supported by the Natural Science Foundation of Guangdong Province (2023A1515012181) .

语种：英文

外文关键词：Bacteriocin; Listeria monocytogenes; Antimicrobial activity; Bacillus velezensis

外文摘要：Listeria monocytogenes, a pathogenic bacterium causing zoonotic diseases, necessitates the urgent search for novel anti-Listeria monocytogenes drugs due to the continuous emergence of drug-resistant bacteria. In this study, we isolated and identified a bacteriocin-producing strain CM7-4 from seawater as Bacillus velezensis through 16S rRNA sequence analysis. Moreover, we successfully purified a novel bacteriocin named PCM7-4 from Bacillus velezensis CM7-4. The molecular weight of PCM7-4 was determined to be 40,228.99 Da. Notably, PCM7-4 exhibited broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria with a minimum inhibitory concentration (MIC) of 5.625 mu g/mL against Listeria monocytogenes specifically. It demonstrated heat resistance and high stability within the pH range of 2-12 while being sensitive to proteinase K degradation without any observed hemolytic activity. Furthermore, SEM analysis revealed that PCM7-4 effectively inhibited biofilm formation and disrupted cell membranes in Listeria monocytogenes cells. Transcriptome analysis revealed that PCM7-4 exerts an impact on genes associated with crucial metabolic pathways, encompassing the biosynthesis of secondary metabolites, phosphotransferase systems (PTS), and starch/sucrose metabolism. These findings highlight the significant potential of bacteriocin PCM7-4 for the development of effective antimicrobial interventions targeting food-borne pathogenic bacteria.