文献类型：期刊文献

英文题名：Mechanism of inactivation of Pseudomonas fluorescens by plasma-activated water based on active substances

作者：Huang, Duanying[1];Sun, Qinchao[3];Meng, Jiaojiao[1];Xu, Jie[1];Pan, Yanmo[1];Zheng, Ouyang[1];Liu, Shucheng[1,2];Sun, Qinxiu[1,2]

机构：[1]Guangdong Ocean Univ, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Guangdong Prov Engn Technol Res Ctr Seafood,Guangd, Coll Food Sci & Technol,Guangdong Prov Engn Lab Ma, Zhanjiang 524088, Peoples R China;[2]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China;[3]Linyi Technician Inst, Linyi 276005, Peoples R China

年份：2024

卷号：96

外文期刊名：INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES

收录：SCI-EXPANDED(收录号：WOS:001280865000001)、、EI(收录号：20243016752457)、Scopus(收录号：2-s2.0-85199128387)、WOS

基金：This work was financially supported by the National Key Research and Development Program of China (2023YFD2401402) .

语种：英文

外文关键词：Plasma-activated water; Treatment condition; Active substances; Pseudomonas fluorescens; Microbial inactivation

外文摘要：The physicochemical properties of plasma-activated water (PAW) and the mechanism of PAW on the inactivation of Pseudomonas fluorescens were investigated. The results demonstrated that as activation time and output voltage increased, the electrical conductivity (EC) and oxidation-reduction potential (ORP) of PAW increased, while its pH decreased. When the activation condition was 20 kV for 30 min (PAW30), the content of active substances of the PAW was the highest (P P < 0.05). The concentrations of NO2, NO3, and H2O2 were high as 0.43 mu g/mL, 2.46 mu g/mL, and 0.837 mu g/mL, respectively. After the PAW30 treatment for 30 min, the population of P. fluorescens was decreased by 2.27 log10 10 CFU/mL when the initial inoculation volume was 4.38 log10(10) CFU/mL. PAW oxidative stress disrupted the balance of the intracellular redox, ion concentration, and pH, leading to the breakdown of cell wall and membrane integrity, ultimately causing bacterial inactivation. These are important mechanisms by which PAW exerts its bactericidal effect. Industrial relevance: The aim of this study was to clarify the optimal preparation conditions for PAW, and its mechanism of killing P. fluorescens. . These results contribute to our understanding of the control of spoilage organisms in aquatic products by PAW. In the raw material treatment stage of aquatic product processing, PAW can be used to clean and soak raw materials to kill microorganisms on their surfaces of raw materials. Therefore, PAW can be applied as a green cleaning technology to improve the hygienic quality of aquatic products.