文献类型：期刊文献

英文题名：Single-cell RNA-seq reveals different subsets of non-specific cytotoxic cells in teleost

作者：Niu, Jinzhong[1];Huang, Yu[1,2,3];Liu, Xinchao[1];Zhang, Zhiqiang[1];Tang, Jufen[1,2,3];Wang, Bei[1,2,3];Lu, Yishan[1,2,3];Cai, Jia[1,2,3];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Guangdong Higher Educ Inst, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Coll Fishery,Key Lab Control Dis Aquat Anim, Zhanjiang, GD, Peoples R China;[2]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;[3]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Asses, Shenzhen, Peoples R China

年份：2020

卷号：112

期号：6

起止页码：5170

外文期刊名：GENOMICS

收录：SCI-EXPANDED(收录号：WOS:000600769000006)、、Scopus(收录号：2-s2.0-85091350684)、WOS

基金：This work was supported by National Natural Science Foundation of China (Grant no. 31302226, 31572651, National Key R&D Program of China (2018YFD0900501), Technology Planning Project of Guangdong Province of China (grant no. 2015A020209181 and 2017A030307033), and Special foundation for "achieving the first class" of Guangdong Province (231419013).

语种：英文

外文关键词：Single cell transcriptome; Non-specific cytotoxic cells; NCC subsets; Innate immune system; Regulatory network

外文摘要：Non-specific cytotoxic cells (NCC) are important cytotoxic leukocytes in teleost immune system. However, the NCC subsets have not been clarified. Thus, we create a comprehensive cell map of similar to 24,062 head kidney-derived leukocytes from Nile tilapia post poly I:C stimulation using single-cell RNA sequencing (scRNA-seq). Based on cell heterogeneity and known markers, the cells were classified into four cell types including B cell, T cell, NCC and monocytes/macrophages (Mo/M Phi). In the meantime, the regulatory network of NCC population was predicted by WGCNA and four hub genes (Stbd1, VWF, PGP, and GRN) and one transcription factor (Hvcn1) were identified. To further study the differentiation of NCC, four subsets including memory-like NCC, mature NCC, immature NCC, and pre-NCC were revealed in NCC population for the first time. Our data will provide new insight into the biology of NCC and enable more accurate functional and developmental analysis of NCC in immune system of lower vertebrates