文献类型：期刊文献

英文题名：Molecular cloning and characterization of calmodulin-like protein CaLP from the Scleractinian coral Galaxea astreata

作者：Huang, Yuanjia[1];Yuan, Jigui[1];Zhang, Yanping[1];Peng, Hiupai[1];Liu, Li[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ An, Zhanjiang, Peoples R China

年份：2018

卷号：23

期号：6

起止页码：1329

外文期刊名：CELL STRESS & CHAPERONES

收录：SCI-EXPANDED(收录号：WOS:000450531000018)、、WOS

基金：This research was funded by the National Marine Welfare Industry Research Project (201105012), Guangdong Provincial Natural Science Foundation (S2011010000269), and Guangdong Marine Fishery Science and Technology Extension Project (A201308E02).

语种：英文

外文关键词：Galaxea astreata; Calmodulin-like protein; Light; Calcium; Temperature stress

外文摘要：Optimal temperature and light are both necessary conditions for coral survival. Light enhances calcification, and thermal stress disrupts Ca2+ homeostasis. As calcium is involved in many important metabolic activities, in this study, we cloned the calmodulin-like protein (CaLP) gene of one of the scleractinian corals, Galaxea astreata. We also detected the relative mRNA expression levels of gaCaLP using the calcium channel blocker verapamil and CaCl2 treatment under conditions of light and dark, and compared expression levels under controlled temperature conditions. Full-length gaCaLP cDNA comprised 1290 nucleotides and contained 498 bp open reading frame that encoded a protein with 165 amino acids. With CaCl2, expression levels of gaCaLP only increased in the presence of light, suggesting that light may be a restrictive factor in CaLP expression when sufficient calcium is available in the environment. In addition, after verapami treatment, we noted that a down regulation of gaCaLP, suggesting that the expression of CaLP is closely related to extracellular Ca2+ influx. Under temperature stress at both high (30 degrees C) and low (20 degrees C) temperatures, expression levels of gaCaLP showed an initial increase, followed by a decreasing trend as treatment progressed. Expression levels reached their maximum value at 24h. This result showed that CaLP participated in a temperature stress response, and Ca2+ homeostasis was disrupted during stress. The findings of the present study will help determine the function and regulatory mechanisms of gaCaLP.