文献类型：期刊文献

中文题名：合浦珠母贝同工酶的电泳分析

英文题名：Analysis on isozymes electrophoresis of Pinctada martensii

作者：李广丽[1];杜晓东[1];叶富良[1]

机构：[1]湛江海洋大学水产学院

年份：2001

卷号：8

期号：2

起止页码：17

中文期刊名：中国水产科学

外文期刊名：Journal of Fishery Sciences of China

收录：CSTPCD、、北大核心2000、Scopus、CSCD2011_2012、北大核心、CSCD

基金：:广东省教育厅重点科技攻关项目

语种：中文

中文关键词：合浦珠母贝;同工酶;多态位点;平均杂合度;电泳

外文关键词：Pinctada martensii; isozymes; electrophoresis; polymorphic loci; heterozygosity

中文摘要：采用聚丙烯酰胺梯度凝胶垂直板电泳技术和特异性染色方法 ,对合浦珠母贝外套膜、闭壳肌、肝脏和鳃组织中的 15种同工酶系统进行分析 ,结果表明 :a GPDH、ADH在 4种组织中均未见表达 ;AK、AMY、PGM、ALP、GDH和LDH只在肝脏中表达 ,而ME、SOD、EST、MDH、G6PD、6PGD和AAT则有较广泛的组织分布 ,其中 12个座位 (Aat 1、Aat 2、Sod 1、Sod 2、Sod 3、Sod 4、m Mdh c、G6pd 2、6Pgd 2、Ak 1、Gdh 1、Me 2 )具有多态性 ,多态位点比例为 4 6.1% ,平均杂合度 0 .112 2± 0 .0 576。

外文摘要：Fifteen isozymes in 4 tissues(gill, adductor muscle, liver and mantle) of Marten's pearl oyster Pinctada martensii were analysed by gradient gel electrophoresis on polyacrytamide and specific dye measures. The results showed that a GPDH and ADH were not found expressing in the 4 tissues; AK, AMY, PGM, ALP, GDH and LDH isozymes only had positive bandsin liver, while MDH, ME, SOD,EST,G6PD, 6PGD and AAT isozymes have wide distribution in the 4 tissues; among those isozymes, 12 loci(Aat 1, Aat 2, Sod 2, Sod 3,Sod 4, m Mdh, G6pd 2, 6Pgd 2, Ak 1, Gdh 1, Me 2, Sod 1) had polymorphisms and the proportion of polymorphic loci(P) was 46.1%, and the average heterozygosity (H) was 0.112 2± 0.057 6.