文献类型：期刊文献

英文题名：Transcriptome analysis of growth heterosis in pearl oyster Pinctada fucata martensii

作者：Yang, Jingmiao[1];Luo, Shaojie[1];Li, Junhui[1];Zheng, Zhe[1];Du, Xiaodong[1,2];Deng, Yuewen[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang, Peoples R China

年份：2018

卷号：8

期号：11

起止页码：1794

外文期刊名：FEBS OPEN BIO

收录：SCI-EXPANDED(收录号：WOS:000449771600005)、、WOS

基金：The research was financially supported by Modern Agricultural Industrial System (CARS-049), Science and Technology Program of Guangdong Province (Grant No. 2015A030302079 and 2017A030303076), Graduate education innovation program of Guangdong Ocean University (201604), Special promotion of fishery science and technology of Ocean and Fisheries Bureau of Guangdong Province (Grant No. B201601-Z09), and 'Innovation Team Project' special funds (2017KCXTD016) from the Department of Education of Guangdong Province.

语种：英文

外文关键词：growth; heterosis; pearl oyster; Pinctada fucata martensii; transcriptome

外文摘要：Heterosis improves growth and survival of shellfish species. Although breeders have widely exploited heterosis, its underlying molecular mechanisms remain unclear. In this study, a 2 x 2 complete diallel cross was facilitated between two full-sib families to produce two inbred families (A and D) and their reciprocal hybrid families (B and C) of pearl oyster Pinctada fucata martensii. Growth traits of the four families were compared at the adult stages. Transcriptome analysis was conducted on the four families using an Illumina sequencing platform. The results revealed that the growth traits of the four families significantly varied (P < 0.05). The mid-parent heterosis values of shell length, shell height, shell width, shell weight, and total weight were 12.9%, 14.9%, 18.2%, 17.2%, and 33.2%, respectively. The B- and C-inbred (A and D) triads had 79 and 68 differentially expressed genes (DEGs), respectively, which were dominantly nonadditive, including overdominance, underdominance, and low-parent dominance. Gene ontology term analysis showed that the DEGs in the B- and C-inbred triads were enriched for metabolic process, cellular process cell part, binding, and catalytic activity. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the DEGs in the B- and C-inbred triads were involved in focal adhesion, the P13K-Akt signaling pathway, the mRNA surveillance pathway, and the focal adhesion pathway. The reliability of the sequencing data was confirmed by real-time polymerase chain reaction analysis of six growth-related genes. The findings of this study provide new insights into heterosis for growth traits and the design of genetic breeding programs for this species.