文献类型：期刊文献

英文题名：Effect of simultaneous variation in temperature and ammonia concentration on percent fertilization and hatching in Crassostrea ariakensis

作者：Wang Hui[1];Liu Jiahui[1];Yang Hongshuai[1];Liu Jin[1];Liu Zhigang[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China

年份：2014

卷号：41

起止页码：43

外文期刊名：JOURNAL OF THERMAL BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000335112700007)、、WOS

基金：The study was financed by the grants from the Guangdong Province Science & Technology Project (No. 20108020201014), the Guangdong Province Education Department (No. GCZX-A0909), the Guangdong Province Ocean and Fisheries Science & Technology Extension Project (No. 20120980) and the Guangdong Province Industry-University-Science Partnership Project (No. 20110908).

语种：英文

外文关键词：Crassostrea ariakensis; Simultaneous variation; Temperature; Ammonia concentration; Fertilization; Hatching

外文摘要：The combined effects of temperature and ammonia concentration on the percent fertilization and percent hatching in Crassostrea ariakensis were examined under laboratory conditions using the central composite design and response surface methodology. The results indicated: (1) The linear effects of temperature and ammonia concentration on the percent fertilization were significant (P < 0.05), and the quadratic effects were highly significant (P < 0.01). The interactive effect between temperature and ammonia concentration on the percent fertilization was not significant (P > 0.05). (2) The linear effect of temperature on the percent hatching was highly significant (P < 0.01), and that of ammonia concentration was nonsignificant (P > 0.05). The quadratic effects of temperature and ammonia concentration on the percent hatching were highly significant (P < 0.01). The interaction on the percent hatching was not significant (P > 0.05). Temperature was more important than ammonia in influencing the fertilization and hatching in C. ariakensis. (3) The model equations of the percent fertilization and hatching towards temperature and ammonia concentration were established, with the coefficients of determination R-2=99.4% and 99.76%, respectively. Through the lack-of-fit test, these models were of great adequacy. The predictive coefficients of determination for the two model equations were as high as 94.6% and 98.03%, respectively, showing that they could be used for practical projection. (4) Via the statistical simultaneous optimization technique, the optimal factor level combination, i.e., 25 C-circle/0.038 mg mL(-1),was derived, at which the greatest percent fertilization 95.25% and hatching 83.26% was achieved, with the desirability being 97.81%. Our results may provide advantageous guidelines for the successful reproduction of C ariakensis. (C) 2014 Elsevier Ltd. All rights reserved.