文献类型：期刊文献

英文题名：Cloning of a novel glutathione S-transferase 3 (GST3) gene and expression analysis in pearl oyster, Pinctada martensii

作者：Chen, Jinhui[1];Xiao, Shu[1];Deng, Yuewen[2];Du, Xiaodong[2];Ye, Ziniu[1]

机构：[1]Chinese Acad Sci, S China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China

年份：2011

卷号：31

期号：6

起止页码：823

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000298569700013)、、Scopus(收录号：2-s2.0-81255135974)、WOS

基金：This work was supported by the national basic research program of China (2010CB126404), national agriculture research funding (nyhyzx07-047) and a China Postdoctoral Science Foundation funded project (20090460835) and a grant from Key Laboratory of Marine Bio-resources Sustainable Utilization, SCSIO (LMB091016). We thank Prof. Elizabeth De Stasio for her editing to the manuscript.

语种：英文

外文关键词：Pearl oyster; MGST; Oxidative stress; Cell defense; Peroxidase activity

外文摘要：Microsomal glutathione S-transferase (MGST) functions in cellular defense against xenobiotics and provides protection against the action of lipid hydroperoxides produced as a consequence of oxidative stress. In this study, a full-length cDNA encoding MGST3 (referred to as PmMGST3) was identified from the pearl oyster. Pinctada martensii by a combination of expressed sequence tag (EST) analysis and rapid amplification of cDNA ends (RACE). The full-length cDNA of PmMGST3 is 971 bp and contains a 5' MR of 39 bp, a 3' UTR of 491 bp with a canonical polyadenylation signal sequence (AATAAA), and an open reading frame (ORF) of 447 bp encoding a polypeptide of 146 residues. The deduced polypeptide contains a conserved motif (FNCx(1)QRx(2)H) characteristic of the MGST3 subfamily. The PmMGST3 transcript could be detected in all tissues tested, with highest transcript level seen in hepatopancreas. Cadmium treatment significantly increased PmMGST3 mRNA levels in gill and hepatopancreas, while bacterial challenge initially depressed mRNA levels and then increased its level in haemocytes, gill and hepatopancreas in a time-dependent manner. In an assay using cumene hydroperoxide as a substrate, we demonstrated that PmMGST3 possesses glutathione-dependent peroxidase activity. These results suggest that PmMGST3 plays an important role in cellular defense against oxidative stress caused by cadmium and bacteria. (C) 2011 Elsevier Ltd. All rights reserved.