文献类型：期刊文献

英文题名：OLE1 reduces cadmium-induced oxidative damage in Saccharomyces cerevisiae

作者：Huang, Zhiwei[1];Yu, Yuanyuan[1];Fang, Zhijia[1,4];Deng, Yunxia[1];Shen, Yuhu[2];Shi, Ping[3]

机构：[1]Donghua Univ, Coll Chem Chem Engn & Biotechnol, Key Lab Sci & Technol Ecotext, Minist Educ, 2999 Renmin Rd, Shanghai 201620, Peoples R China;[2]Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Key Lab Crop Mol Breeding Qinghai Prov, Xining 810008 23, Peoples R China;[3]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, 1 Haida Rd, Mazhang Dist 524088, Zhanjiang, Peoples R China

年份：2018

卷号：365

期号：18

外文期刊名：FEMS MICROBIOLOGY LETTERS

收录：SCI-EXPANDED(收录号：WOS:000449462200008)、、WOS

基金：This work was sponsored by grants from National Natural Science Foundation of China (31671309 and 31701706), the Open Project of Qinghai Provincial Key Laboratory of Crop Molecular Breeding (2017-ZJ-Y14).

语种：英文

外文关键词：OLE1; cadmium; oxidative stress; antioxidation; Saccharomyces cerevisiae

外文摘要：Cadmium has been shown to be an important environmental pollutant. Our previous studies have shown that the regulation of OLE1 in the synthesis of unsaturated fatty acids may act as a positive feedback mechanism to help yeast cells counter the lipid peroxidation and cytoplasmic membrane damage induced by cadmium. However, the involvement of OLE1 in cadmium-induced oxidative stress is still unclear. In this study, we explored the effects of OLE1 on cadmium-induced oxidative stress in yeast. Different from ascorbic acid, the overexpression of OLE1 did not affect the activities of superoxide dismutase, catalase and glutathione peroxidase. Although OLE1 overexpression induced an increase in GSH levels, the anti-oxidative mechanism of OLE1 was GSH1 independent. On the other hand, similar to ascorbic acid, OLE1 overexpression significantly reduced the levels of superoxide radical, carbonyl protein and lipid peroxidation. Additionally, overexpression of OLE1 effectively prevented cell membrane damage induced by cadmium. OLE1 could also reduce the cytotoxicities of other heavy metals stress, including copper, tri-and hexavalent chromium. Thus, our results indicate that overexpression of OLE1 reduces oxidative stress induced by cadmium possibly through the inhibition of lipid peroxidation and protection of the cytoplasmic membrane from damage in yeast cells. Furthermore, we determined that the anti-oxidative effect of OLE1 is independent of antioxidant enzymes and GSH1.