文献类型：期刊文献

英文题名：Quantitative Label-Free Listeria Analysis Based On Aptamer Modified Nanoporous Sensor

作者：Zhou, Chun-Xia[1];Mo, Ri-Jian[1];Chen, Zhi-Meng[1];Wang, Juan[1];Shen, Guo-Zhu[1];Li, Yi-Ping[1];Quan, Qin-Guo[1];Liu, Ying[1];Li, Cheng-Yong[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China

年份：2016

卷号：1

期号：8

起止页码：965

外文期刊名：ACS SENSORS

收录：SCI-EXPANDED(收录号：WOS:000385465200002)、、WOS

基金：This work was supported by the Natural Science Foundation of China (21405024), Science and Technology Planning Project of Guangdong Province (2014A020212683, 2016A020210114), Science and Technology Planning Project of Zhanjiang City (2015A03025), Foundation for Outstanding Young Teachers of Guangdong Ocean University (2014005), Training Programs of Innovation and Entrepreneurship for Undergraduates (201510566003).

语种：英文

外文关键词：aptamer; Listeria monocytogenes; steric blockage; charge repulsion; electrochemical detection; anodic aluminum oxide

外文摘要：In this Letter, we proposed a nanoporous sensor to realize a quick and sensitive detection of Listeria monocytogenes with the application of porous anodic aluminum oxide membrane combined with a specific aptamer. On the basis of electrochemical detection, it can be known that the reduction current response upon the diffusion flux of electrochemical probe is affected by the charge repulsion and steric blockage of Listeria monocytogenes. A rapid detection can be achieved within 10 min, with a high sensitivity of 100 CFU/mL and a good linear range between 100 to 1250 CFU/mL. Moreover, it has a high specificity for Listeria monocytogenes even exposed in 10(8) CFU/mL Staphylococcus aureus or Escherichia coli.