文献类型：期刊文献

中文题名：饲料n-3 HUFA水平对珍珠龙胆石斑鱼幼鱼生长、免疫力及相关基因表达和抗病力的影响

英文题名：Effects of dietary n-3 highly unsaturated fatty acids on growth, immunity and related gene expression and disease resistance of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatu)

作者：安文强[1,2];董晓慧[1,2];谭北平[1,2];杨奇慧[1,2];迟淑艳[1,2];章双[1,2];刘泓宇[1,2];杨原志[1]

机构：[1]广东海洋大学水产学院,水产动物营养与饲料实验室,广东湛江524088;[2]广东省水产动物精准营养与高效饲料工程技术研究中心,广东湛江524088

年份：2019

卷号：43

期号：10

起止页码：2123

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、北大核心2017、Scopus、CSCD2019_2020、北大核心、CSCD

基金：国家自然科学基金(31972808);现代农业产业技术体系专项(CARS-47);广东海洋大学“冲一流”省财政专项(231419011)~~

语种：中文

中文关键词：珍珠龙胆石斑鱼;n-3高不饱和脂肪酸;非特异性免疫;炎症相关基因

外文关键词：Epinephelus fuscoguttatus♀×Epinephelus lanceolatu♂;n-3 HUFA;non-specific immunity;inflammatory related gene

中文摘要：本实验旨在研究饲料中不同n-3 高不饱和脂肪酸(HUFA)水平对珍珠龙胆石斑鱼幼鱼生长性能、非特异性免疫、免疫相关基因表达及对抵抗哈维氏弧菌能力的影响。配制n-3 HUFA水平分别为0.65%(对照组)、1.00%、1.35%、1.70%、2.05%和2.40%的6种等氮等脂的实验饲料,投喂初始体质量为(12.06 ± 0.01) g的珍珠龙胆石斑鱼幼鱼8周。结果显示:①饲料n-3 HUFA水平对饲料系数(FCR)、存活率(SR)、肝体比(HSI)和肥满度(CF)均无显著性影响;1.35%组增重率(WGR)和特定生长率(SGR)显著高于2.40%组。②1.00%组的体粗脂肪含量显著低于1.70%和2.40%组。③攻毒前,1.35%和1.70%组血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性和补体C3的浓度显著高于对照组。攻毒后,血清CAT、谷胱甘肽过氧化物酶(GSH-Px)、溶菌酶(LZM)活性和C3含量急剧上升,SOD活性显著下降。攻毒前,2.40%组肠道TLR22和MyD88 mRNA的表达量显著增加,2.05%组TNF-α和IL-1β的表达量显著高于1.00%和1.35%组;此外,1.70%组肾脏TLR22和IL-1β的表达量显著低于对照组。攻毒后,1.35%组肠道MyD88 mRNA的表达量显著高于1.00%和1.70%~2.40%组,1.70%组TNF-α和IL-1β的表达量有最小值;1.70%组肾脏IL-10的表达量显著高于其他各组,而IL-1β的表达量呈相反趋势,显著低于2.40%组。研究表明,适宜的饲料n-3 HUFA(1.47%~1.70%)可以提高珍珠龙胆石斑鱼的生长性能和非特异性免疫力,并抑制促炎基因的表达。

外文摘要：This study was conducted to investigate the effects of dietary n-3 highly unsaturated fatty acids (n-3 HUFA) on growth performance, non-specific immunity, expression of some immune-related genes and resistance to Vibrio harveyi in juvenile hybrid grouper (Epinephelus fuscoguttatus ♀× E. lanceolatu♂). Six isoproteic and isolipidic experimental diets were formulated with graded levels of n-3 HUFA (0.65%, 1.00%, 1.35%, 1.70%, 2.05% and 2.40% of dry matter, respectively), and 0.65% group was used as control group. Each diet was randomly allocated to triplicate groups of fish in 1 000 L fiberglass tank, and each tank was stocked with 40 fish [initial weight (12.06 ± 0.01) g] for 8 weeks. Results showed that feed conversion ratio (FCR), survival rate (SR), hepatosomatic index (HSI) and condition factor (CF) were not significantly affected by dietary n-3 HUFA levels. Weight gain (WG) and specific growth rate (SGR) in 1.35% group were significantly higher than those in 2.40% group. Crude lipid of body in 1.00% group was significantly lower than those in 1.70% and 2.40% groups Liver and muscle fatty acid profiles reflected that of diets. Before challenge, the activity of serum superoxide dismutase (SOD), catalase (CAT) and content of complement 3 (C3) in 1.35% and 1.70% groups significantly higher than those of control group. After challenge, serum CAT, glutathione peroxidase (GSH-Px), lysozyme (LZM) and C3 all increased sharply, while SOD showed the opposite trend. Before challenge, the expression levels of intestine toll-like receptor 22 (TLR22) and myeloid differentiation factor 88 (MyD88) mRNA in 2.40% group were significantly increased, and the expression levels of tumour necrosis factor α(TNF-α) and interleukin 1β(IL-1β) mRNA in 2.05% group were significantly higher than those in 1.00% and 1.35% groups. In addition, the TLR22 and IL-1β mRNA levels in kidney of 1.70% group were significantly lower than those in control group. After challenge, the expression level of MyD88 mRNA in intestine of 1.35% group was significantly higher than that in 1.00% group and from 1.70% to 2.40% group, while TNF-α and IL-1β obtained minimum values in 1.70% group. In the kidney, the interleukin 10 (IL10) mRNA expression was significantly higher in 1.70% group than those in other groups, while the IL-1β expression in 1.70% group showed the opposite trend and significantly lower than that in 2.40% group. Results of this study suggested that moderate dietary n-3 HUFA (1.47%-1.70% HUFA) could improve the growth performance, non-specific immunity and inhibit the inflammatory response of hybrid grouper.