文献类型：期刊文献

英文题名：Modeling inhibitory activity of a novel antimicrobial peptide AMPNT-6 from Bacillus subtilis against Vibrio parahaemolyticus in shrimp under various environmental conditions

作者：Pu, Yuehua[1];Sun, Lijun[1];Wang, Yaling[1];Qi, Deng[1];Chen, Danmin[1];Liu, Huanming[1];Xu, Defeng[1];Deng, Chujing[1];Li, Jianrong[2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod,Guangdong Higher Educ, Zhanjiang 524088, Guangdong, Peoples R China;[2]Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Food Safety Key Lab Zhejiang Prov, Hangzhou 310035, Zhejiang, Peoples R China;[3]Bohai Univ, Food Safety Key Lab Liaoning Prov, Jinzhou 121013, Peoples R China

年份：2013

卷号：33

期号：1

起止页码：249

外文期刊名：FOOD CONTROL

收录：SCI-EXPANDED(收录号：WOS:000319032300037)、、WOS

基金：The authors gratefully acknowledge the National Science Fund (No. 30279987) and the National Key Technologies R & D Program of China during the period of the 12th Five-Year Plan (Key technology of quality and safety control during aquatic product processing, No. 2012BAD29B06) for their financial supports.

语种：英文

外文关键词：Antimicrobial peptides; Shrimps; Vibrio parahaemolyticus; Mathematical model

外文摘要：Vibrio parahaemolyticus is recognized as the leading cause of human gastroenteritis associated with the consumption of seafood. NT-6 antimicrobial peptide (AMPNT-6) that is a novel antimicrobial peptide secreted by Bacillus subtilis NT-6 isolated from Natto which is a Chinese traditional fermented food can be an effective method to reduce V. parahaemolyticus. This study aimed at investigating the factors that affected the application of AMPNT-6, such as temperature, salinity, pH value and sodium pyrosulfite concentration by response surface method and modeled the effect of AMPNT-6 on inhibition to V. parahaemolyticus in shrimp under various environmental conditions. Design-Expert software was chosen to perform regression fit and to establish the antibiotic mathematical model. The results indicated that all the above environmental conditions had no significant interactive effects on the antibacterial activity of AMPNT-6. The quadratic polynomial mathematical model was established and obtained the quadratic regression equation of the predictive value Y (bacterial colony number) and variables x(1) (temperature), x(2) (sodium chloride concentration), x(3) (pH), x(4) (sodium pyrosulfite concentration) and x(5) (AMPNT-6 concentration) in a certain range: Y = 6.60 + 0.34x(1) - 0.23x(3) - 0.19x(5) - 0.29x(1)(2) + 0.19x(2)(2) + 03x(4)(2) - 035x(1)x(2) (R-2 = 0.8963). The experimental values were shown to be in good agreement with predicted values. The established model had a high fitting degree and could predict the inhibition effect of AMPNT-6 toward V. parahaemolyticus growth in shrimp under various conditions. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.