文献类型：期刊文献

英文题名：A novel bacteriocin from Lacticaseibacillus rhamnosus with potent anti-Vibrio activity: Purification, characterization and application in shrimp preservation

作者：Li, Guanglan[1];Zeng, Lanwei[1];Li, Junke[1];Zhang, Haolin[1];Khan, M. R.[3];Brennan, Charles[4];Pei, Jinjin[1,2];Abd El-Aty, A. m.[5,6]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Engn Technol Res Ctr Prefabricated, Coll Food Sci & Technol,Guangdong Prov Engn Lab Ma, Guangdong Prov Engn Technol Res Ctr Seafood,Guangd, Zhanjiang 524088, Peoples R China;[2]Ataturk Univ, Fac Agr, Dept Food Engn, TR-25240 Erzurum, Turkiye;[3]Univ Agr Swat, Dept Food Sci & Technol, Swat Mingora 19130, Pakistan;[4]Univ Otago, Dept Food Sci, Dunedin 9016, New Zealand;[5]Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt;[6]Ataturk Univ, Fac Med, Dept Med Pharmacol, TR-25240 Erzurum, Turkiye

年份：2027

卷号：193

外文期刊名：FOOD CONTROL

收录：SCI-EXPANDED(收录号：WOS:001890733700001)、、WOS

基金：This study was funded by the Talent Startup Funding of Guangdong Ocean University (060302042404) and the Foreign Expert Project of the Ministry of Science and Technology (S20260368) .

语种：英文

外文关键词：Lacticaseibacillus rhamnosus; Bacteriocin; Antibacterial mechanism; Vibrio parahaemolyticus; Shrimp

外文摘要：The increasing prevalence of antibiotic-resistant pathogens, particularly in seafood, necessitates the discovery of novel biopreservatives. This study reports the isolation of a Lacticaseibacillus rhamnosus SLG21 strain from fermented fish sauce, which exhibits exceptional tolerance to high selenite concentrations (5 mM) and potent antagonistic activity against foodborne pathogens. A novel bacteriocin, designated SLG21, was purified and identified as a 16-residue cationic peptide (KYGNGGLSRIFSALLK, 1554.15 Da). Bacteriocin SLG21 demonstrated broad-spectrum bactericidal activity, with minimum inhibitory concentrations (MICs) of 4-16 mu g/mL against Gram-positive pathogens and, crucially, 16-128 mu g/mL against Gram-negative pathogens. Mechanistic studies revealed that the bacteriocin SLG21 overcomes the Gram-negative outer membrane barrier through high-affinity binding to LPS, leading to outer membrane permeabilization and subsequent inner membrane depolarization. Toxicology assessments confirmed its biosafety at preservative-relevant concentrations. In a shrimp model inoculated with Vibrio parahaemolyticus, bacteriocin SLG21 (1 & times; MIC) rapidly inhibited bacterial growth and delayed chemical spoilage (as measured by total volatile basic nitrogen). Although its recoverable activity decreased to 28.5% by day 8, the initial bactericidal action may suggest potential application in Pacific white shrimp against V.parahaemolyticus.