文献类型：期刊文献

英文题名：Whole Transcriptome Analysis Reveals the Global Molecular Responses of mRNAs, lncRNAs, miRNAs, circRNAs, and Their ceRNA Networks to Salinity Stress in Hong Kong Oysters, Crassostrea hongkongensis

作者：Ibrahim, Salifu[1];Yang, Chuangye[2,3];Yue, Chenyang[1];Song, Xinyu[1];Deng, Yuewen[2,3];Li, Qi[4];Lu, Wengang[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Guangdong Marine Invertebrates Sci & Technol Innov, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Engn Lab Mariculture Organism Breed, Zhanjiang 524088, Peoples R China;[4]Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao 266003, Peoples R China

年份：2023

卷号：25

期号：4

起止页码：624

外文期刊名：MARINE BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001037123200001)、、EI(收录号：20233014451254)、Scopus(收录号：2-s2.0-85165934274)、WOS

基金：This study was supported by grants from the Guangdong Basic and Applied Basic Research Foundation (2022A1515110002), the Science and Technology Program of Zhanjiang City (2021E05029), the Chinese Ministry of Science and Technology through the National Key Research and Development Program of China (2018YFD0901401), the Department of Education of Guangdong Province (2021KCXTD026), the project of the Innovation Team for the Innovation and Utilization of Economic Animal Germplasm in the South China Sea (2021KCXTD026), and the program for scientific research start-up funds of Guangdong Ocean University (060302022105).

语种：英文

外文关键词：Salt stress; Crassostrea hongkongensis; Whole transcriptome analysis; CeRNA

外文摘要：The Hong Kong oyster, Crassostrea hongkongensis, is an estuarine bivalve with remarkable commercial value in South China, and the increase of salinity in estuaries during the dry season has posed a major threat to the oyster farming. To explore the global transcriptional response to salinity stress, a whole-transcriptome analysis was performed with the gills of oysters in 6 & PTSTHOUSND;, 18 & PTSTHOUSND;, and 30 & PTSTHOUSND; filtered seawater. Overall, 2243, 194, 371, and 167 differentially expressed mRNAs (DEmRNAs), differentially expressed long non-coding RNAs (DElncRNAs), differentially expressed circular RNAs (DEcircRNAs), and differentially expressed microRNAs (DEmiRNAs) were identified, respectively. Based on GO enrichment and KEGG pathway analysis, these important DEmRNAs, DElncRNAs, DEcircRNAs, and DEmiRNAs were predicted to be mainly involved in amino acids metabolism, microtubule movement, and immune defense. This demonstrated the complexity of dynamic transcriptomic profiles of C. hongkongensis in response to salinity fluctuation. The regulatory relationships of DEmiRNAs-DEmRNAs, DElncRNAs-DEmiRNAs, and DEcircRNAs-DEmiRNAs were also predicted, and finally, a circRNA-associated competing endogenous RNA (ceRNA) network was constructed, consisting of six DEcircRNAs, eight DEmiRNAs, and five DEmRNAs. The key roles of taurine and hypotaurine metabolism and phenylalanine metabolism were highlighted in this ceRNA network, which was consistent with the major contribution of free amino acids to intracellular osmolality and cell volume regulation. Collectively, this study provides comprehensive data, contributing to the exploration of coding and non-coding RNAs in C. hongkongensis salinity response. The results would benefit the understanding of the response mechanism of bivalves against salinity fluctuation, and provide clues for genetic improvement of C. hongkongensis with hyper-salinity tolerance.