详细信息
A sensitive fluorometric sensor for Ag+based on the hybridization chain reaction coupled with a glucose oxidase dual-signal amplification strategy ( SCI-EXPANDED收录) 被引量:2
文献类型:期刊文献
英文题名:A sensitive fluorometric sensor for Ag+based on the hybridization chain reaction coupled with a glucose oxidase dual-signal amplification strategy
作者:Li, Yubin[1];Xie, Ling[1];Yuan, Jiaming[1];Liu, Huazhong[1]
机构:[1]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China
年份:2020
卷号:10
期号:44
起止页码:26239
外文期刊名:RSC ADVANCES
收录:SCI-EXPANDED(收录号:WOS:000548745800023)、、WOS
基金:This work is supported by the project of Enhancing School with Innovation of Guangdong Ocean University, China (no. 2020KZDZX1108), the foundation and applied foundation research Joint-Youth Fund Project of Guangdong Province, China (no. 2019A1515110648), the Youth Innovation Talents Project of Guangdong Province Universities (no. 2017KQNCX096), and the Science and Technology Project on Special Fund for Public Welfare Research and Ability Construction of Guangdong Province (no. 2017A010105010).
语种:英文
外文摘要:In this work, an efficient and sensitive fluorometric sensor was developed to detect silver ions (Ag+). It is based on the cytosine-Ag+-cytosine (C-Ag+-C) structureviaa dual-signal amplification strategy using glucose oxidase (GOx) and the hybridization chain reaction (HCR). A silver-coated glass slide (SCGS) acts as an ideal material for separation. Cytosine rich (C-rich) capture DNA (C-DNA) assembled themselves on the SCGSviaAg-S bonds and hybridized with signal DNA (S-DNA) to trigger the HCR. With specific base-pairing, the S-DNA and HCR products bind on the SCGS. Then, the GOx-biotin-streptavidin (SA) complexes bind to the HCR products through SA-biotin interactions. Owing to the formation of a particular C-Ag+-C structure between two neighboring C-rich C-DNA on the SCGS, the C-DNA/S-DNA/HP1-GOx/HP2-GOx complex gradually moved away from the SCGS as the concentration of Ag(+)increased and the combined GOx fell into the buffer. H(2)O(2)could be generated during the oxidation of glucose, catalyzed by GOx in the buffer. Afterward, H(2)O(2)could oxidize the substrate (3-(p-hydroxyphenyl)-propanoic acid) when Horseradish peroxidase was present, giving rise to blue fluorescence. The proposed strategy reached a limit of detection (LOD) of 1.8 pmol L(-1)with a linear detection range of 5 to 1000 pmol L(-1)for Ag+. Moreover, this assay has been commendably used for the detection of Ag(+)in actual samples with fairly good results.
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