文献类型：期刊文献

中文题名：蟹肉蛋白对小鼠系统性低度炎症的缓解作用及其与肠道菌群和短链脂肪酸的相关性

英文题名：The Alleviating Effect of Crab Meat Protein on Systemic Low-grade Inflammation in Mice and I ts Correlation with Gut Microbiota and Short-chain Fatty Acids

作者：黄铃[1];何晓彤[1];胡连花[1];王晨[1];骆津津[1];房志家[1];邓旗[1];孙力军[1]

机构：[1]广东海洋大学食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东湛江524088

年份：2025

卷号：41

期号：11

起止页码：27

中文期刊名：现代食品科技

外文期刊名：Modern Food Science and Technology

收录：北大核心2023、、北大核心

基金：广东省现代农业产业技术体系创新团队建设专项(2023KJ149;2023KJ151)。

语种：中文

中文关键词：系统低度炎症;蟹肉蛋白;短链脂肪酸;肠道菌群失调

外文关键词：systemic low-grade inflammation;crab meat protein;short chain fatty acids;disorder of gut microbiota

中文摘要：为探究蟹肉蛋白(CMP)对系统低度炎症小鼠的调节作用及其与肠道菌群和短链脂肪酸(SCFAs)的相关性。本研究将小鼠随机分为空白组、模型组、鱼油组和低、中、高剂量CMP组,干预7 d后测定小鼠血清IL-6、IL-2、IL-1β、TNF-α、脂多糖(LPS)、粪便SCFAs水平和肠道菌群结构组成。结果显示,模型组血清LPS、IL-6、IL-2、IL-1β、TNF-α水平显著升高220.52%、79.21%、28.51%、114.30%、111.92%,肠道中毛螺菌属(Lachnospiraceae_NK4A136_group)、拟杆菌属(Bacteroides)、乳杆菌属(Lactobacillus)相对丰度和粪中丁酸、戊酸、异丁酸含量显著降低73.99%、54.02%、68.31%、59.51%、30.49%、7.49%。CMP可使小鼠血清炎性因子、肠道菌群多样性及丰富度基本恢复至空白组水平,产SCFAs菌的相对丰度和粪丁酸、戊酸、异丁酸含量显著升高,且干预效果优于IM组。据相关性分析,丁酸、戊酸与IL-2呈负相关,异丁酸与IL-1β呈负相关,而毛螺菌属与戊酸、丁酸呈正相关,拟杆菌属与丁酸呈正相关,乳杆菌属与异丁酸呈正相关。综上所述,CMP可缓解辣椒素诱导的系统低度炎症,该缓解作用与肠道菌群及其代谢产物丁酸、异丁酸和戊酸有关。

外文摘要：In order to explore the regulatory effects of crab meat protein(CMP)on systemic low-grade inflammation in mice and its correlation with gut microbiota and short-chain fatty acids(SCFAs),in this study,mice were randomly divided into a blank(control)group,a model group,a fish oil group,and low-,medium-,and high-dose CMP groups.After 7 days of intervention,serum levels of IL-6,IL-2,IL-1β,TNF-α,lipopolysaccharide(LPS),fecal SCFA concentrations,and the composition of gut microbiota were determined.The results showed that compared with the blank group,the model group’s serum LPS,IL-6,IL-2,IL-1β,and TNF-αlevels were significantly elevated by 220.52%,79.21%,28.51%,114.30%,and 111.92%,respectively,and their relative abundances of Lachnospiraceae_NK4A136_group,Bacteroides,and Lactobacillus,and fecal butyric acid,valeric acid,and isobutyric acid contents were significantly reduced by 73.99%,54.02%,68.31%,59.51%,30.49%and 7.49%,respectively.CMP administration restored serum inflammatory factors,gut microbiota diversity and richness to the levels almost the same as those of the blank group,while increasing significantly the relative abundance of SCFA-producing bacteria and the contents of fecal butyric acid,valeric acid,and isobutyric acid.The intervention of the CMP outcome was better than that of IM.According to correlation analysis,butyric acid and valeric acid were negatively correlated with IL-2,whilst isobutyric acid was negatively correlated with IL-1β.Furthermore,Lachnospiraceae_NK4A136_group showed positive correlations with valeric acid and butyric acid,Bacteroides exhibited a positive correlation with butyric acid,and Lactobacillus displayed a positive correlation with isobutyric acid.In conclusion,CMP can alleviate systemic low-grade inflammation induced by capsaicin,and this alleviation is associated with the gut microbiota and its metabolites butyric acid,isobutyric acid,and valeric acid.