文献类型：期刊文献

英文题名：Berberine Hydrochloride Impact on Physiological Processes and Modulation of Twist Levels in Nasopharyngeal Carcinoma CNE-1 Cells

作者：Li, Cai-Hong[1,2];Wu, Dong-Fang[1];Ding, Hang[2];Zhao, Yang[3];Zhou, Ke-Yuan[2];Xu, De-Feng[4]

机构：[1]Wuhan Univ, Zhongnan Hosp, Wuchang, Peoples R China;[2]Guangdong Med Coll, Institude Biochem & Mol Biol, Dongguan, Peoples R China;[3]Guangdong Med Coll, Affiliated Hosp 2, Ear Nose Throat Dept, Zhanjiang, Peoples R China;[4]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China

年份：2014

卷号：15

期号：4

起止页码：1851

外文期刊名：ASIAN PACIFIC JOURNAL OF CANCER PREVENTION

收录：SCI-EXPANDED(收录号：WOS:000333670100063)、、Scopus(收录号：2-s2.0-84896968701)、WOS

基金：This study was financially supported by grants from the National Natural Science Foundation of China (Project codes: GZ0602, 81272434), The Dongguan Science and Technology Bureau (Project code: DK0912). The authors are grateful for the support of the Key Laboratory of Medical Molecular Activity Research and would like to express thanks to Mrs. Xiuwen Si for her help with the Twist gene expression study.

语种：英文

外文关键词：Berberine hydrochloride; invasion; migration; apoptosis; cell proliferation; CNE-1

外文摘要：Objective: The main purpose of this work was to investigate the effect of berberine hydrochloride (BH) on the proliferation, apoptosis, migration, and invasion of CNE-1 nasopharyngeal carcinoma cells. Our results shed light on the functional components of traditional Chinese herbs for potential use in modern medicine. Methods: The CNE-1 cell line was treated with different concentrations of BH and effects on cell viability and proliferation were evaluated using the Cell Counting Kit-8 (CCK-8) assay. Anti-migratory and anti-invasive actions of BH were investigated using wound healing assays and the Millicell Hanging cell culture insert system, respectively. Expression of the epithelial-mesenchymal transition (EMT)-related gene twist (Twist) was analyzed by real-time PCR and Western blotting. Apoptosis was estimated with an annexin-V fluorescein (FITC) apoptosis detection kit, as well as with reference to levels of activated caspase-3 of CNE-1 cells before and after treatment with BH utilizing fluorescence spectroscopy. Results: BH was capable of reducing proliferation and viability of CNE-1 cells in a dose-and time-dependent manner, also demonstrating anti-migratory and anti-invasive capacities which correlated with reduction in expression of Twist. Finally, BH was able to induce significant amounts of apoptosis in CNE-1 cells, as demonstrated by an increase in the activity of caspase-3 and in annexin-V staining following treatment. Conclusion: BH extracted from rhizoma coptidis demonstrated an ability to block proliferation, induce apoptosis, and impair the migration and invasion of the CNE-1 cell line Considering these properties, our results suggest that BH could be an important compound for consideration in the treatment of nasopharyngeal carcinoma.