文献类型：期刊文献

英文题名：Protective role of l-threonine against cadmium toxicity in Saccharomyces cerevisiae

作者：Huang, Linru[1];Fang, Zhijia[1];Gao, Jian[1];Wang, Jingwen[1];Li, Yongbin[1];Sun, Lijun[1];Wang, Yaling[1];Liao, Jianmeng[2];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Cunjin Coll,Guangdong Prov Key Lab Aquat Prod Pro, Guangdong Prov Engn Technol,Res Ctr Marine Food, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pr, Zhanjiang, Peoples R China;[2]Inst Food & Drug Control, Zhanjiang, Peoples R China;[3]Lincoln Univ, Dept Wine Food & Mol Biosci, Canterbury, New Zealand

年份：2021

卷号：61

期号：4

起止页码：339

外文期刊名：JOURNAL OF BASIC MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000616899000001)、、WOS

基金：Characteristic Innovation Project of Guangdong Province, Grant/Award Number: 2018KTSCX089; National Natural Science Foundation of China, Grant/Award Number: 31701706; Program for Scientific Research Start-up Funds of the Guangdong Ocean University, Grant/Award Number: R17102; Natural Science Foundation of Guangdong Province, Grant/Award Number: 2019A1515010809

语种：英文

外文关键词：cadmium; cadmium tolerance; metal toxicity; Saccharomyces cerevisiae; yeast

外文摘要：Environment and food contamination with cadmium (Cd) can cause serious toxicity, posing a severe threat to agricultural production and human health. However, how amino acids contribute to defenses against oxidative stress caused by Cd in cells is not fully understood. As a model eukaryote with a relatively clear genetic background, Saccharomyces cerevisiae has been commonly used in Cd toxicity research. To gain insight into Cd toxicity and cell defenses against it, 20 amino acids were screened for protective roles against Cd stress in S. cerevisiae. The results showed that threonine (Thr, T) had the strongest protective effect against Cd-induced mortality and membrane damage in the cells. Compared to the antioxidant vitamin C (VC), Thr exhibited a higher efficacy in restoring the superoxide dismutase (SOD) activity that was inhibited by Cd but not by H2O2 in vivo. Thr exhibited evident DPPH (2,2-diphenyl-1-picrylhydrazyl) activity but weak ABTS (2,2 '-azino-bis(3-ethylbenzothiazoline-6-9 sulfonic acid)) scavenging activity, giving it a weaker effect against Cd-induced lipid peroxidation and superoxide radical O2-, compared to VC. More importantly, compared to the chelating agent EDTA, Thr showed stronger chelation of Cd, giving it a stronger protective effect on SOD against Cd than VC in vitro. The results of the in vivo and in vitro experiments revealed that the role Thr plays in cell defenses against Cd may be attributed to its protection of the SOD enzyme, predominantly through the preferential chelation of Cd. Our results provide insights into the protective mechanisms of amino acid Thr that ameliorate Cd toxicity and suggest that a supplement of Thr might help to reduce Cd-induced oxidative damage.