文献类型：期刊文献

中文题名：凡纳滨对虾内源优势腐败菌的分离和鉴定

英文题名：Separation and identification of endogenous dominant spoilage bacteria from Litopenaeus vannamei

作者：叶日英[1];孙力军[1];王雅玲[1];何国炜[1];徐德峰[1];刘唤明[1];张永平[1]

机构：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室,广东湛江524025

年份：2013

卷号：37

期号：9

起止页码：1425

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、北大核心2011、Scopus、北大核心、CSCD、CSCD2013_2014

基金：国家自然科学基金项目(30972287,31201309);“十二五”国家科技支撑计划(2012BAD29B06);广东省教育部产学研结合项目(2011B090400154)

语种：中文

中文关键词：凡纳滨对虾;内源优势腐败菌;分离;鉴定

外文关键词：Litopenaeus vannamei;endogenous dominant spoilage bacteria;separation;indentification

中文摘要：为了确定凡纳滨对虾在冷藏条件下内源优势腐败菌的种类,实验采用了细菌分离纯化法,将分离得到的内源腐败菌菌株进行分解蛋白质对比实验,筛选出分解蛋白质能力较强的菌株,进一步采用细菌自动生化系统鉴定法以及16S rRNA序列测定法进行鉴定,实验结果显示凡纳滨对虾在冷藏条件下的内源优势腐败菌是短杆菌属细菌,可以通过控制短杆菌属细菌的方法进行研究对虾内源保鲜技术。细菌自动生化系统鉴定结果的相似度为98.8%,16S rRNA序列鉴定结果的相似度为99.065%。

外文摘要：Endogenous spoilage bacteria of Litopenaeus vannamei are the bacterial floras living in Litopenaeus vannamei body which cause the Litopenaeus vannamei spoilage.It is one of the most important factors which cause spoilage while the Litopenaeus vannamei are stored up and in transportation process.In order to find out the endogenous dominant spoilage bacteria of Litopenaeus vannamei under refrigeration,the bacteria separation is applied in experiment and therefore the endogenous spoilage bacteria are gained,and then the bacterial strains of high capability of decomposing protein were obtained after protein comparison experiment.Once more,auto-biochemical identification system and 16S rRNA sequencing are applied in experiment process.The result shows that the endogenous dominant spoilage bacteria of Litopenaeus vannamei are of Brevibacterium,and the semblance of auto-biochemical identification and 16S rRNA sequencing is 98.833% and 99.065% respectively.Researches show that the endogenous dominant spoilage bacteria of Litopenaeus vannamei under refrigeration are of Brevibacterium,and it is possible to research on endogenous freshness preservation technology of Litopenaeus vannamei by controlling Brevibacterium bacteria.