文献类型：期刊文献

英文题名：Ammonia Toxicity Induces Oxidative Stress, Inflammatory Response and Apoptosis in Hybrid Grouper (♀ Epinephelus fuscoguttatus x ♂ E. lanceolatu)

作者：Yan, Xiaobo[1,2];Chen, Yu[1,2];Dong, Xiaohui[1,2,3];Tan, Beiping[1,2,3];Liu, Hao[1,2];Zhang, Shuang[1,2,3];Chi, Shuyan[1,2,3];Yang, Qihui[1,2,3];Liu, Hongyu[1,2,3];Yang, Yuanzhi[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Nutr & Feed, Zhanjiang, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang, Guangdong, Peoples R China

年份：2021

卷号：8

外文期刊名：FRONTIERS IN MARINE SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000642803900001)、、WOS

基金：This study was supported financially by the National Key R&D Program of China (Grant No. 2019YFD0900200), National Natural Science Foundation of China (Grant No. NSFC31972808), Postgraduate Education Innovation Project of Guangdong Ocean University (Grant No. 202028), Science and Technology Bureau of Zhanjiang (Grant Nos. 2020A03010 and 2020A05003), Agriculture Research System of China (Grant No. CARS-47), and "Chong Yi Liu" project of Guangdong Ocean University (Grant No. 231419011).

语种：英文

外文关键词：ammonia poisoning; serum biochemistry; non-specific immunity; genes; intestinal microbial; half-lethal concentrations

外文摘要：To study the effects of acute ammonia stress on the poisoning reaction of the hybrid grouper (? Epinephelus fuscoguttatus ? ? E. lanceolatu), 300 healthy grouper juveniles with an initial body weight of 51.4 ? 2.57 g were selected for an acute ammonia stress experiment using a half-lethal concentration of ammonia for 24, 48, 72, and 96 h with triplicate. The results show that (1) The half-lethal concentrations of ammonia for a hybrid grouper were 39.5 mg/L for 24 h, 27.3 mg/L for 48 h, 26.5 mg/L for 72 h, and 25.0 mg/L for 96 h, and the safe concentration was 2.50 mg/L. (2) The biochemical indices of the serum and livers of the groupers fluctuated under acute ammonia stress (P < 0.05), and the contents of serum cortisol and lactate reached the maximum value in 72 h (P < 0.05). (3) 24 h ammonia stress increased the activities of alkaline phosphatase, acid phosphatase, catalase, glutathione peroxidase, superoxide dismutase, lysozyme, and the content of immunoglobulin M in the liver (P < 0.05). Meanwhile, all ammonia-stressed fish groups had an increased amount of malondialdehyde in the liver (P < 0.05). (4) The ammonia poisoning caused significantly up-regulation of antioxidant genes, inflammatory cytokines, and apoptosis genes (P < 0.05), and the expression of inflammatory cytokines and apoptosis genes were the highest in 24 h ammonia stress group. (5) The ammonia content in the water changed the abundance and evenness of intestinal microbes in grouper, mainly in that ammonia stress significantly increased the relative abundance of fusobacteria, but significantly decreased the relative abundance of gemmatimonadetes (P < 0.05). It was speculated that apoptosis induced by ammonia poisoning in grouper may be related to oxidative stress and the activation of inflammatory factors, and excessive inflammatory stress may be one of the causes of ammonia poisoning in the hybrid grouper.