文献类型：期刊文献

英文题名：Effects of T-2 Toxin on Pacific White Shrimp Litopenaeus vannamei: Growth, and Antioxidant Defenses and Capacity and Histopathology in the Hepatopancreas

作者：Deng, Yijia[1];Wang, Yaling[1];Zhang, Xiaodi[1];Sun, Lijun[1];Wu, Chaojin[1];Shi, Qi[1];Wang, Rundong[1];Sun, Xiaodong[2];Bi, Siyuan[3];Gooneratne, Ravi[4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Dalian Nationalities Univ, Coll Environm & Resources, Dalian 116600, Peoples R China;[3]Shenzhen Bioeasy Biotechnol Co Ltd, Shenzhen 518102, Peoples R China;[4]Lincoln Univ, Ctr Food Res & Innovat, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand

年份：2017

卷号：29

期号：1

起止页码：15

外文期刊名：JOURNAL OF AQUATIC ANIMAL HEALTH

收录：SCI-EXPANDED(收录号：WOS:000395096300002)、、Scopus(收录号：2-s2.0-85014930714)、WOS

基金：This study was supported financially by the National Natural Science Foundation of China (NSFC) (numbers 31171634, 31371777, and 31200014), the Science and Technology Planning Project of Guangdong Province (number 2014B020205006), the Guangdong Oceanic and Fishery Administration Project (Guangdong Fiscal Government on Agriculture [2015] number 115), an Enhancing Innovation School Project of Guangdong Ocean University (numbers 2013050205, 2014050203, and GDOU2013050312), and a Shenzhen Technology Research and Development Foundation Project (GCZX201405091631511273).

语种：英文

外文摘要：Modified-masked T-2 toxin (mT-2) formed during metabolism in edible aquatic animals may go undetected by traditional analytical methods, thereby underestimating T-2 toxicity. The effects of T-2 on growth and antioxidant capacity and histopathological changes in the hepatopancreas were studied in Pacific white shrimp Litopenaeus vannamei exposed for 20 d to 0, 0.5, 1.2, 2.4, 4.8, and 12.2 mg/kg of T-2 in their feed. The concentration of mT-2 in the hepatopancreas was detected by liquid chromatography-tandem mass spectrophotometry before and after trifluoroacetic acid (TFA) treatment that converted mT-2 to free T-2. A dose-dependent increase in mT-2 concentration was observed in the hepatopancreas. Dietary exposure to T-2 significantly decreased (P < 0.05) shrimp growth and survival rate compared with the controls. The malondialdehyde (MDA) concentration was significantly increased in shrimp exposed to feed with >= 2.4 mg/kg T-2 (P < 0.05). The antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx), total antioxidant capacity (T-AOC), and also glutathione (GSH) content increased in shrimp dosed with 2.4-4.8mg/kgT-2 but declined at the highest dose (12.2 mg/kg), probably indicating an inability to cope with high concentrations of reactive oxygen species (ROS) as evident from a marked increase in MDA (P < 0.05) culminating in cellular toxicity. Histopathological changes in the hepatopancreaswere dose dependent, with cell autophagy evident at the highest exposure dose. This is the first report in shrimp of a dose-dependent increase in ROS, SOD enzyme activity, and T-AOC at low T-2 exposures, and associated histopathological changes in the hepatopancreas, in response to dietary T-2.