文献类型：期刊文献

英文题名：Protective mechanisms of three antioxidants against T-2 toxin-induced muscle protein deterioration in shrimp

作者：Huang, Xiaoyue[1];Huang, Zhanrui[1,2];Sun, Lijun[1];Qiu, Mei[1];Deng, Qi[1];Fang, Zhijia[1];Wang, Yaling[1]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Coll Food Sci & Technol,Guangdong Prov Engn Techn, Guangdong Prov Engn Lab Marine Biol Prod,Key Lab, Zhanjiang, Peoples R China;[2]Shaoyang Univ, Coll Food & Chem Engn, Hunan Prov Key Lab Soybean Prod Proc & Safety Con, Shaoyang, Peoples R China

年份：2022

卷号：102

期号：11

起止页码：4883

外文期刊名：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

收录：SCI-EXPANDED(收录号：WOS:000768664600001)、、Scopus(收录号：2-s2.0-85126218853)、WOS

基金：This work was supported by the National Natural Science Foundation of China (NSFC) (no. 31871898), Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams (no. 2021KJ149 and no. 2021KJ151), Characteristic Innovation Project of Colleges and Universities in Guangdong Province (no. 2020KQNCX025), Zhanjiang Competitive Allocation Project of Science and Technology Development Special Fund (no. 200915134541577 and no. 2020A03009) and Demonstration and Popularization of Shrimp Seed Industry and Supporting Technology.

语种：英文

外文关键词：Litopenaeus vannamei; T-2 toxin; antioxidants; protective mechanism; muscle protein

外文摘要：BACKGROUND Quercetin (Q), tea polyphenols (TP), and rutin (R) are widely used plant-derived active ingredients. They possess antioxidant, anti-inflammatory, and anti-tumor properties, and can reduce the muscle damage caused by mycotoxins. However, few studies have examined the protective mechanisms of quercetin, tea polyphenols, and rutin on muscle quality. To elucidate their protective mechanisms, shrimp were exposed to both T-2 toxin and these three antioxidants for 20 days in a dose-escalating trial. The changes in the protein composition of shrimp muscle were measured. The target proteins associated with T-2 and antioxidants were screened and identified by non-labeled quantitative proteomics. RESULTS The T-2 toxin induced abnormal expression of 21 target proteins, leading to the deterioration of muscle proteins in shrimp. The three antioxidants ameliorated the T-2 toxin-induced damage to muscle proteins by increasing the sarcoplasmic and myofibrillar protein content and decreasing the alkali-soluble protein content. Quercetin had the strongest protective effect. The protective processes of these antioxidants involved the upregulation of target proteins involved in carbohydrate metabolism (enolase, malate dehydrogenase), protein translation (elongation factor 1-alpha and eukaryotic translation initiation factor 2 subunit alpha), and cytoskeleton component (actin 2, fast-type skeletal muscle actin 1). Quercetin regulated the largest number of target proteins, making it the best protective agent against T-2 toxin. CONCLUSION The T-2 toxin (4.80-24.30 mg/kg feed) induced changes in target proteins and muscle composition of shrimp, leading to a deterioration in muscle proteins. Quercetin (2.00-32.00 g/kg feed) had significant protective effects against this deterioration in muscle protein in shrimp. (c) 2022 Society of Chemical Industry.