文献类型：期刊文献

英文题名：Combination of amplified rDNA restriction analysis and high-throughput sequencing revealed the negative effect of colistin sulfate on the diversity of soil microorganisms

作者：Fan, Tingli[1,4];Sun, Yongxue[2];Peng, Jinju[1];Wu, Qun[1];Ma, Yi[1];Zhou, Xiaohui[3,5,6]

机构：[1]Guangdong Ocean Univ, Agr Coll, Dept Vet Med, Zhanjiang 524088, Peoples R China;[2]South China Agr Univ, Guangdong Key Lab Vet Pharmaceut Dev & Safety Eva, Guangzhou 510642, Guangdong, Peoples R China;[3]Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Jiangsu Key Lab Zoonosis, Yangzhou 225009, Jiangsu, Peoples R China;[4]Cangzhou Tech Coll, Dept Anim Husb & Vet Med, Cangzhou 061001, Peoples R China;[5]Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT 06269 USA;[6]Yangzhou Univ, Key Lab Prevent & Control Biol Hazard Factors Ani, Minist Educ China, Joint Int Res Lab Agr & Agri Prod Safety, Yangzhou 225009, Jiangsu, Peoples R China

年份：2018

卷号：206

起止页码：9

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000423013700002)、、EI(收录号：20173804186639)、Scopus(收录号：2-s2.0-85029491123)、WOS

基金：This work was partially supported by Guangdong Provincial Natural Science Foundation of China (2017A030313133), Guangdong Provincial Key Natural Science Foundation of China (To Y. Ma) (2016A030311029) and National Natural Science Foundation of China (To X. Zhou) (31772741).

语种：英文

外文关键词：Colistin sulfate; Veterinary medicine; Ecological toxicity; Microbial diversity

外文摘要：Colistin sulfate is widely used in both human and veterinary medicine. However, its effect on the microbial ecologyis unknown. In this study, we determined the effect of colistin sulfate on the diversity of soil microorganisms by amplified rDNA restriction analysis (ARDRA) and high-throughput sequencing ARDRAshowed that the diversity of DNA from soil microorganisms was reduced after soil was treated with colistin sulfate, with the most dramatic reductionobserved after 35 days of treatment. High-throughput sequencing showed that the Chaol and abundance-based coverage estimators (ACE) Were reduced in the soils treated with colistin sulfate for 35 dayscompared to those treated with colistin sulfate for 7 days. Furthermore, Chaol and ACE tended to be lower when higher concentration of colistin sulfate was used, suggesting that the microbial abundance is reduced by colistin sulfate in a dose-dependent manner. Shannon index showed that the diversity of soil microorganism was reduced upon treatment with colistin sulfate compared to the untreated control group. Following 7 days of treatment, Bacillus, Clostridiumand Sphingomonas were sensitive to all the concentration of colistin sulfate used in this study. Following 35 days of treatment, the abundance of Choroplas4 Haliangium, Pseudomonas, Lactococcus, and Clostridium was significantly decreased. Our results demonstrated that colistin sulfate especially at high concentration (>= 5 mg/kg) could alter the population structure of microorganisms and consequently the microbial community function in soil.