文献类型：期刊文献

英文题名：Isolation of a putative probiotic strain S12 and its effect on growth performance, non-specific immunity and disease-resistance of white shrimp, Litopenaeus vannamei

作者：Liu, Hongyu[1];Li, Zheng[2];Tan, Beiping[1];Lao, Ye[2];Duan, Zhiyong[2];Sun, Wuwei[1];Dong, Xiaohui[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524025, Peoples R China;[2]Kemin Ind Zhuhai Co Ltd, Zhuhai 519110, Peoples R China

年份：2014

卷号：41

期号：2

起止页码：300

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000347017500022)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (Nos. 30871928, 31272673), the National Basic Research Program of China (2014CB138600), the Special Fund for Agro-scientific Research of Public Interest (No. 201003020), and the High Level Talent Project of Guangdong Province Universities and Colleges Pearl River Scholar (GDUPS) (2011).

语种：英文

外文关键词：Probiotics; Bacillus subtilis; Pathogen; Litopenaeus vannamei; Immunity

外文摘要：The common pathogens in aquaculture are very different from those in terrestrial animals. The objective of this study was to isolate probiotic strain (s) from the digestive tract of healthy white shrimp Litopenaeus vannamei which was effective against aquatic animal pathogens. The putative probiotic strain S12 was identified as Bacillus subtilis based on the morphological and biochemical properties and 16S rDNA gene sequencing. The L. vannamei were fed with five different diets: control (basal diet with no probiotics or antibiotics), antibiotic control (basal diet supplemented with 0.3% florfenicol), basal diet supplemented with 5 x 10(9) cfu kg(-1) 5 x 10(10) cfu kg(-1) and 5 x 10(11) cfu kg(-1) probiotic S12 (PS1-3). Each diet was randomly fed to quadruplication groups of 40 shrimps (0.4 +/- 0.01 g) reared in tanks. After an 8-week feeding, the survival rate of shrimps fed with PS1 and PS3 were the highest among all treatments (P < 0.05). The moisture content of shrimps fed with florfenicol was significantly lower than that of the control group (P < 0.05). The supplement of probiotic S12 decreased the body crude lipid significantly (P < 0.05). The activities of phagocytic rate, lysozyme (LZ), superoxide dismutase phenoloxidase (SOD) and antibacterial activity were significantly higher than those in the control (P < 0.05), and the activities of SOD and the antibacterial activity in PS2 and PS3 were significantly higher than those in antibiotic control (P < 0.05). When infected with Vibrio harveyi at 4-weeks, the mortality was significantly lower (P < 0.05) in PS2 and PS3 groups than that in the control. After being infected with V. harveyi at 8-weeks, the mortality was significantly lower in the probiotic and antibiotic groups than that in the control (P < 0.05). This study suggested that probiotics could be used as an effective immunopotentiator, the optimal dose of the probiotic strain S12 is 5 x 10(10) cfu kg(-1) diet. (C) 2014 Elsevier Ltd. All rights reserved.