文献类型：期刊文献

中文题名：急性碱胁迫对金钱鱼肝脏和鳃组织结构及转录组响应的影响

英文题名：Effects of acute alkali stress on the structural and transcriptomic responses of liver and gill tissues in Scatophagus argus

作者：杨磊[1];田昌绪[1,2,3];彭友琦[1];刘勇[1];陈宇[1];段存煜[1];韩彤[1];施钢[1,2,3];朱春华[1,2,3];李广丽[1,2,3]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]广东海洋大学,广东省名特优鱼类生殖调控与繁育工程技术研究中心,广东湛江524088;[3]广东海洋大学,广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088

年份：2025

卷号：49

期号：11

起止页码：72

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：北大核心2023、、北大核心

基金：新疆维吾尔自治区重点研发任务专项(2024B02014-3);湛江市科技计划项目(2022A01046);国家自然科学基金(32172971);广东省自然科学基金(2021A1515010430);广东海洋大学本科生创新团队项目(CXTD2023003);广东省南美白对虾现代种业产业园项目(K22221)。

语种：中文

中文关键词：金钱鱼;碱胁迫;组织结构;转录组测序;氧化应激

外文关键词：Scatophagus argus;alkali stress;organizational structure;transcriptome sequencing;oxidative stress

中文摘要：【目的】明确金钱鱼对碱度胁迫的应答机制。【方法】设置对照组(淡水)和碱胁迫实验组(30 mmol/L碳酸盐碱度),分别在胁迫0、3、6和9 h后,对金钱鱼的肝脏和鳃组织进行了组织结构观察和转录组测序。【结果】组织切片结果显示,在碱胁迫下,金钱鱼肝细胞出现不同程度的萎缩和空泡化现象,鳃组织也表现出上皮细胞脱落、鳃小片变形及间距变大等损伤特征;转录组测序结果显示,碱胁迫处理9 h后,在肝组织中共有270个差异表达基因,其中190个上调,80个下调。这些基因主要涉及细胞色素P450相关代谢、脂肪细胞因子信号通路和类固醇代谢等与能量合成和代谢相关通路。其中,hsp70和hbb1的上调及gpx1的下调表明氧化应激响应机制的启动。在鳃组织中检测到114个差异表达基因,其中39个上调,75个下调,主要影响了细胞黏附、类固醇生物合成、PPAR信号等通路。prkca和rab5a的显著下调表明细胞黏附能力可能受到损害。此外,肝脏和鳃组织中共同差异表达基因ck和nlrp12在免疫防御方面起关键作用。【结论】金钱鱼对碱胁迫的响应涉及肝脏和鳃组织的结构和功能变化,并通过调节与能量平衡和免疫反应相关的基因表达来作出应答。这一发现为进一步解析金钱鱼耐碱性的分子机制提供了重要信息。

外文摘要：To elucidate the response mechanisms of Scatophagus argus to carbonate alkalinity stress,we established a control group in freshwater and an experimental group exposed to 30 mmol/L carbonate alkalinity.Liver and gill tissues were collected at 0,3,6,and 9 hours post-stress for histological examination and transcriptome sequencing.Histological analysis revealed that under alkaline stress,liver cells exhibited varying degrees of atrophy and vacuolization,while gill tissues demonstrated damage characterized by epithelial cell shedding,gill lamella deformation,and larger spacing.Transcriptome sequencing after 9 hours of alkaline stress identified 270 differentially expressed genes(DEGs)in liver tissue,with 190 genes up-regulated and 80 downregulated.These DEGs were primarily associated with pathways related to energy synthesis and metabolism,including cytochrome P450-related metabolism,adipocytokine signaling,and steroid metabolism.Notably,the up-regulation of hsp70 and hbb1,alongside the down-regulation of gpx1,indicated the activation of oxidative stress response mechanisms.In gill tissue,114 DEGs were identified,with 39 up-regulated and 75 down-regulated,affecting pathways such as cell adhesion,steroid biosynthesis,and PPAR signaling.The significant down-regulation of prkca and rab5a suggested a potential compromise in cell adhesion capacity.In addition,the common differentially expressed genes ck and nlrp12 in liver and gill tissues play a key role in immune defense.These findings indicate that the adaptation of S.argus to carbonate alkalinity stress involves structural and functional alterations in liver and gill tissues,along with the regulation of gene expression related to energy balance and immune response.This study provides critical insights into the molecular mechanisms underlying alkali resistance in S.argus.