文献类型：期刊文献

英文题名：Transcriptome analysis of liver provides insight into metabolic and translation changes under hypoxia and reoxygenation stress in silver sillago (Sillago sihama)

作者：Tian, Changxu[1];Lin, Xinghua[1];Saetan, Wanida[1];Huang, Yang[1];Shi, Hongjuan[1];Jiang, Dongneng[1];Chen, Huapu[1];Deng, Siping[1];Wu, Tianli[1];Zhang, Yulei[1];Li, Guangli[1];Zhu, Chunhua[1]

机构：[1]Guangdong Ocean Univ, Fisheries Coll,Southern Marine Sci & Engn Guangdo, Guangdong Res Ctr Reprod Control & Breeding Techn, Guangdong Prov Engn Lab Mariculture Organism Bree, Zhanjiang 524088, Peoples R China

年份：2020

卷号：36

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

收录：SCI-EXPANDED(收录号：WOS:000591917100001)、、WOS

基金：This study was supported by grants from the National Natural Science Foundation of China (Nos. 41706174 and 31702326); Natural Science Foundation of Guangdong Province (2016A030313743, 2017A030313101, 2018B030311050, 2019A1515010958, 2019A1515110619); Independent Project of Guangdong Province Laboratory (ZJW-2019-06); the Department of Education of Guangdong Province (2018KQNCX111, 2019KTSCX060) and Program for Scientific Research Start-up Funds of Guangdong Ocean University (R19026).

语种：英文

外文关键词：Transcriptome; Sillago sihama; Hypoxia stress; Liver; Gene expression

外文摘要：Hypoxia can lead to adverse effects on growth, reproduction, behavioral activities and survival in fish, and is one of the most critical factors in the aquatic environment. The liver is an important target organ for reducing toxin accumulation and hypoxia in fish. In this study, silver sillago (Sillago sihama) was exposed to normoxia (dissolved oxygen, DO = 8.0 mg/L), hypoxia for 1 h (hypoxia 1 h, DO = 1.5 mg/L), hypoxia for 4 h (hypoxia 4 h, DO = 1.5 mg/L) and reoxygenation for 4 h after hypoxia 4 h (reoxygenation 4 h, DO = 8.0 mg/L). Results showed that the expression of 506, 1721, and 1230 differentially expressed genes (DEGs) (vertical bar log(2)(fold change) > 1.0 vertical bar and padj < 0.05) were identified at hypoxia 1 h, hypoxia 4 h, and reoxygenation 4 h in the liver, respectively. The enrichment analysis showed that the DEGs were significantly enriched in metabolic and translation changes pathways, including mapk signaling pathway, p53 signaling pathway, fatty acid metabolism, protein export, ribosome biogenesis in eukaryotes. The DEGs of 17 genes validated the RNA-seq results by quantitative real-time PCR (qRT-PCR). This study provides a comprehensive understanding of the transcriptional changes that occur in different hypoxia and insights into the mechanisms of hypoxia adaptation of the liver in S. sihama.