文献类型：期刊文献

英文题名：Trash to treasure: Potential antifreeze peptide from Litopenaeus vannamei head via ultrasound-assisted autolysis

作者：Tan, Mingtang[1,2,3,4];Han, Mei[1];Zhou, Yingyu[1];Chen, Zhongqin[1,2,3,4];Cao, Wenhong[1,2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Natl Res & Dev Branch Ctr Shellfish Proc Zhanjiang, Zhanjiang 524088, Peoples R China;[3]Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China

年份：2025

卷号：27

外文期刊名：FOOD CHEMISTRY-X

收录：SCI-EXPANDED(收录号：WOS:001455483700001)、、EI(收录号：20251318110321)、Scopus(收录号：2-s2.0-105000620028)、WOS

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 32172163) and the Program for scientific research start-up funds of Guangdong Ocean University (060302042314) .

语种：英文

外文关键词：Shrimp head autolysate; Antifreeze peptides; Bioinformatic; Molecular dynamics simulation; Antifreeze mechanism

外文摘要：This study aimed to separate and purify antifreeze peptides (AFP) from the autolysate of Litopenaeus vannamei head, and the peptide sequences with antifreeze activity were identified to elucidate the potential antifreeze mechanisms. The initial fractionation of autolysate revealed that the pk1 fraction with less than 3 kDa molecular weight exhibited the highest thermal hysteresis activity (2.28 degrees C). Notably, the component pk1-A with the strongest antifreeze activity (2.80 degrees C) was further separated by using a Sephadex G-15 gel filtration column. The results of bioinformatics and computer-assisted techniques indicated that 26 types of peptides from pk1-A were identified as AFP. Among these, KQVHPDTGISSK was selected as a potential Litopenaeus vannamei head antifreeze peptide (LvAFP). The active site (Lys residue) of LvAFP was discovered to strongly interact with water molecules via hydrogen bonding, thereby inhibiting the formation and recrystallization of ice crystals. Therefore, the preparation of LvAFP could improve the high-value utilization of shrimp byproducts.