文献类型：期刊文献

英文题名：Acetylcholinesterase inhibitors and antioxidants mining from marine fungi: bioassays, bioactivity coupled LC-MS/MS analyses and molecular networking

作者：Nie, Yingying[1,2];Yang, Wencong[2];Liu, Yayue[2];Yang, Jingming[2];Lei, Xiaoling[2];Gerwick, William H.[3];Zhang, Yi[1,2,3,4]

机构：[1]Guangdong Ocean Univ, Ctr Marine Drugs Res & Dev, Shenzhen Inst, Shenzhen 518000, Peoples R China;[2]Guangdong Ocean Univ, Res Inst Marine Drugs & Nutr,Key Lab Adv Proc Aqu, Coll Food Sci & Technol,Guangdong Prov Engn Techn, Guangdong Prov Engn Lab Marine Biol Prod,Key Lab, Zhanjiang 524088, Peoples R China;[3]Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA;[4]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2020

卷号：2

期号：4

起止页码：386

外文期刊名：MARINE LIFE SCIENCE & TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000649454300007)、、Scopus(收录号：2-s2.0-85112423255)、WOS

基金：This work was supported by the Yangfan Talent Project of Guangdong Province under Grant 201433009; the Natural Science Foundation of Guangdong Province under Grant 2018A030307046; the Basic Research Project of Shenzhen Science and Technology Innovation Commission under Grant JCYJ20190813105005619; the Shenzhen Dapeng New District Industrial Development Fund under Grants KY20180203 & PT201901-05; the Shenzhen Dapeng New District Scientific and Technological Research and Development Fund under Grant KJYF202001-07; the Natural Science Foundation of China under Grant 21807015; the Program for Scientific Research Start-Up Funds of Guangdong Ocean University under Grant E15155; the Applied S & T Research and Development Major Project of Guangdong Province under Grant 2016B020235001; and the Scientific Fund of the Zhanjiang Municipal Oceanic and Fishery Bureau under Grant A18018; the Project of Enhancing School with Innovation of Guangdong Ocean University (230420022). We also thank E. Glukhov and L. Gerwick for helpful discussions (Scripps Institution of Oceanography, University of California, San Diego).

语种：英文

外文关键词：Acetylcholinesterase inhibition; Antioxidant; Marine fungi; LC-MS/MS; Molecular networking

外文摘要：Marine fungi are potentially important resources for bioactive lead compounds for discovering new drugs for diseases such as Alzheimer's disease. In this paper, the combined bioassay model of acetylcholinesterase (AChE) inhibition, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and Artemia larval lethality was used to evaluate the activity and toxicity of 35 marine fungal strains from seas around China. Their bioactive constituents were revealed by thin layer chromatography (TLC) autography, bioactivity coupled LC-MS/MS and Global Natural Products Social Molecular Networking (GNPS). The results show that the extracts of five strains exhibited higher AChE inhibition ratios than the positive control compound, 'tacrine', for which the ratio was 89.8% at 200 mu g/ml. Six strains displayed both AChE inhibition (inhibition ratios > 20% at 200 mu g/ml) and DPPH scavenging activity (scavenging ratios > 30% at 200 mu g/ml) together with low Artemia larval toxicity (lethal rates < 12%). TLC autography showed that the fractioned extracts of four strains contained highly diverse and different bioactive constituents, including strains Talaromyces sp. C21-1, Aspergillus terreus C23-3, Trichoderma harzianum DLEN2008005, and Penicillium corylophilum TBG1-17. From the most potent sample F-11-1-b (derived from Aspergillus terreus C23-3), five AChE inhibitors and seven antioxidants were recognized as bioactive molecules by AChE coupled ultrafiltration followed by LC-MS/MS, and LC-MS/MS coupled with DPPH incubation. Furthermore, with the aid of GNPS, the AChE inhibitors were plausibly annotated as territrem analogues including territrems A-C/D, arisugacin A and an unknown compound 4, and the seven antioxidants were assigned as butyrolactone., aspernolide E, a phenolic derivative and possibly unknown compounds 8-10 and 12.