文献类型：期刊文献

英文题名：The synthetic progestin norethindrone causes thyroid endocrine disruption in adult zebrafish

作者：Liang, Yan-Qiu[1];Xu, Wenqiang[1];Liang, Xingyi[1];Jing, Zhanxin[1];Pan, Chang-Gui[2];Tian, Fei[3]

机构：[1]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[2]Guangxi Univ, Guangxi Lab Study Coral Reefs South China Sea, Nanning 530004, Peoples R China;[3]Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China

年份：2020

卷号：236

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

收录：SCI-EXPANDED(收录号：WOS:000557992800012)、、Scopus(收录号：2-s2.0-85085916985)、WOS

基金：This work was funded by the National Natural Science Foundation of China (NSFC 41907346), Natural Science Foundation of Guangdong Province (2017A030310662, 2019A1515011889, 2017A030313220).

语种：英文

外文关键词：Progestin; Norethindrone; Hypothalamus-pituitary-thyroid axis; Zebrafish; Thyroid hormone

外文摘要：Synthetic progestin norethindrone (NET) widely used in oral contraceptives, hormonal therapy and livestock farming has been detected in various aquatic ecosystems. Recent studies have shown that NET can cause thyroid endocrine disruption in amphibians. However, studies are still lacking on thyroid axis of fish. In the present study, we investigated thyroid hormone levels (T3 and T4) and transcriptional patterns of 15 genes of the hypothalamic-pituitary-thyroid axis (HPT axis) in adult zebrafish that were exposed to solvent control and three measured concentrations of NET (7, 84 and 810 ng/L) for 90 days. The results indicated that NET significantly lowered T3 and T4 levels in both female and male zebrafish. Transcriptional expression profiles of some of the HPT-axis related genes were disrupted. Specifically, the expression levels of tshb and pax8 have increased significantly while dio2 and ugt1ab have decreased in females. In male, however, tshb expression levels were increased while ttr, ugt1ab, thra and thrb were decreased. The overall results demonstrate that NET disrupts thyroid endocrine system by interfering at multiple sites along HPT axis in adult zebrafish.