文献类型：期刊文献

中文题名：乌贼墨多糖提取工艺优化

英文题名：Optimization of extraction of polysaccharide from cuttlefish ink

作者：吴金龙[1];刘华忠[2];师莉莎[1];王光[1];黄燕[2];钟杰平[3];潘江球[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]广东海洋大学现代生化中心,广东湛江524088;[3]广东海洋大学理学院,广东湛江524088

年份：2011

卷号：32

期号：7

起止页码：289

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、CSCD2011_2012、北大核心2008、北大核心、CSCD

语种：中文

中文关键词：乌贼墨多糖;提取;黑色素

外文关键词：cuttlefish ink polysaccharide; extraction ; melanin

中文摘要：通过单因素实验和正交实验,对乌贼墨多糖的提取工艺进行了研究。主要考察了不同料液比、pH、提取温度以及提取时间对多糖得率及含量的影响,同时比较了活性炭吸附和高速离心法对乌贼墨黑色素的去除效果。实验结果表明:高速离心法去除黑色素方法优于活性炭吸附;乌贼墨多糖最佳提取工艺条件为:料液比为1∶2,pH为5,提取温度为40℃,提取时间为4h,此条件下乌贼墨多糖得率为1.228%,糖含量为78%,初步纯化后蛋白脱除率为90.52%,多糖的损失率为12.45%。

外文摘要：Extraction of the cuttlefish ink polysaccharide was studied through single- factor experiment and orthogonal test.The ratio of material to liquid,pH,temperature and extracting time were studied by extraction rates of polysaccharides.AIso activated carbon adsorption and high-speed centrifugation on the removal of the effect of Sepia melanin was compared.The results showed that activated carbon adsorption was better than high-speed centrifugation,the optimum conditions of extraction squid ink polysaccharide was.liquid ratio 1：2, pH5 ,temperature 40℃ and 4h for extracting, at this point the yield of polysaccharides was 1.228% ,the content of polysaccharides was 78%After purified, protein removal rate was 90.52% ,the loss rate of polysaccharides was 12.45%.