文献类型：期刊文献

中文题名：无乳链球菌抑菌中草药的筛选及其穿透尼罗罗非鱼体血脑屏障能力的研究

英文题名：A study on the ability of Brazilin to penetrate the blood-brain barrier of Nile tilapia(Oreochromis niloticus)in vitro

作者：郑梦婕[1];李梓昕[1];陈杨慧[1];蔡佳[1];黄瑜[1];汤菊芬[1];牟希东[2];杨叶欣[2];王蓓[1]

机构：[1]广东海洋大学水产学院、广东省水产动物病害防控与健康养殖重点实验室水产经济动物病害控制广东省普通高校重点实验室,广东湛江524088;[2]中国水产科学研究院珠江水产研究所农业农村部外来入侵水生生物防控重点实验室,广东广州510380

年份：2025

卷号：40

期号：3

起止页码：372

中文期刊名：大连海洋大学学报

外文期刊名：Journal of Dalian Ocean University

收录：北大核心2023、、北大核心

基金：国家自然科学基金(32073006);农业农村部外来入侵水生生物防控重点实验室2023年开放基金(20230302)。

语种：中文

中文关键词：尼罗罗非鱼;血脑屏障;无乳链球菌;巴西苏木素;LC-MS

外文关键词：Oreochromis niloticus;blood-brain barrier;Streptococcus agalactiae;Brazilian;LC-MS A UPLC-MS/MS

中文摘要：为研究巴西苏木素(Brazilin)穿透血脑屏障(blood-brain barrier,BBB)的能力,探究巴西苏木素治疗罗非鱼脑部疾病的可行性,筛选抑菌效果良好的中草药苏木,建立了测定尼罗罗非鱼(Oreochromis niloticus)血脑屏障体外模型中巴西苏木素的UPLC-MS/MS分析方法。研究表明:试验中使用的36味中草药中,苏木水提物对无乳链球菌(Streptococcus agalactiae)的体外抑菌效果最好,选择其有效成分巴西苏木素进行后续试验;CCK8试验结果显示,在试验浓度范围内,巴西苏木素对构成血脑屏障的罗非鱼脑内皮细胞(TVEC-01)和罗非鱼星形胶质细胞(TA-02)生长无明显抑制作用;浓度为20、40、80μmol/L的巴西苏木素均能下调紧密连接蛋白JAM-B、ZO-1、Claudin-5的表达水平(P<0.05);给药30 min后即可在血脑屏障模型下池样品中检测到巴西苏木素,且穿透浓度随给药浓度的增加而增加。结果表明,巴西苏木素能透过血脑屏障发挥抑菌作用,可为筛选治疗罗非鱼脑部疾病的药物提供科学参考。

外文摘要：To explore the ability of Brazilin to penetratethe blood-brain barrier and its potential to treat brain diseases in tilapia,a UPLC-MS/MS analytical method was established for the determination of Brazilin in an in vitro model of the blood-brain barrier of the Nile tilapia(Oreochromis niloticus).The antibacterial efficacy of 36 different Chinese herbal medicines against Streptococcus agalactiae(ZQ0910)was assessed by using the agar diffusion method,and the Minimum Inhibitory Concentration of these extracts was determined using a two-fold dilution technique.Herbs exhibiting significant antibacterial activity were selected for a subsequent in vitro permeability experiment to penetrate blood-brain barrier.The effects of an antibacterial agent on the proliferation of tilapia brain endothelial cells(TVEC-01)and tilapia astrocyte cells(TA-02)were determined using the CCK8 method in a co-culture model of TVEC-01 and TA-02 cells.The effects of bacteriostatic drugs on the expression levels of the tight junction-associated proteins JAM-B,ZO-1,and Claudin-5 in the in vitro blood-brain barrier were then detected using qRT-PCR.Finally,the concentrations of the bacteriostatic drugs in pooled samples from the in vitro blood-brain barrier model were determined using UPLC-MS/MS.The experimental results showed that of the 36 herbs tested in the experiment,the sappan wood aqueous extract had the greatest in vitro inhibitory effect on S.lactis.The results of CCK8 experiment showed that Brazilin in the range of experimental concentrations had no significant inhibitory effect on the growth of the TVEC-01 and TA-02 cells constituting the blood-brain barrier.Concentrations of 20,40,and 80μmol/L of Brazilin all regulated down the expression levels of tight junction proteins JAM-B,ZO-1,and Claudin-5(P<0.05).Brazilin could be detected in samples from the lower pool of blood-brain barrier model after 30-minute administration,and the penetration concentration increased with increase in concentration of drug administered.These findings demonstrate that Brazilin can penetrate the blood-brain barrier and exert antibacterial effects,and provide a feasible strategy for screening drugs to treat brain diseases.