文献类型：期刊文献

中文题名：光镜及扫描电镜下波纹龙虾血淋巴细胞的形态及其分类

英文题名：Classification and morphological observation of haemocytes of Panulirus homarus by LM and SEM

作者：黄翔鹄[1];李长玲[1];刘楚吾[1]

机构：[1]湛江海洋大学水产学院

年份：2004

卷号：28

期号：6

起止页码：645

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、北大核心2000、Scopus、CSCD2011_2012、北大核心、CSCD

基金：湛江海洋大学自然科学研究项目(0312023)

语种：中文

中文关键词：波纹龙虾;血淋巴细胞;显微结构;扫描电镜

外文关键词：Panulirus homarus; hemocyte;microstructure;scanning electron microscope

中文摘要：光镜及扫描电镜下,研究波纹龙虾血淋巴细胞的组织学结构、形态学特征和数量。根据细胞质中颗粒存在与否,颗粒数量的多少,细胞体积的大小及外部形态,对波纹龙虾的血淋巴细胞进行分类。结果表明,光镜下波纹龙虾的血淋巴细胞有3种类型:无颗粒细胞,小颗粒细胞,大颗粒细胞;这3种血淋巴细胞不是连续发育过程中血淋巴细胞的不同阶段,而是彼此独立的。3种血淋巴细胞的数量占循环血淋巴细胞总量的比例分别为:无颗粒细胞占57.1%,大颗粒细胞占24.9%,小颗粒细胞占18.0%;在扫描电镜下,细胞表面不平,有各种突起,揭示细胞具有活跃的变形运动和吞噬能力。血淋巴细胞形态有球形、卵圆形和椭圆形。

外文摘要：The kind and quantity of hemocytes in crustaceans are very important for immune defense. There is prominent difference of immune mechanism in the different species and types of hemocyte of crustaceans. It is very important to study the form structure and function of hemocyte for practice. Histology structure, morphology characters and quantity of hemocyte of Panulirus homarus are studied by light microscope(LM) and scanning electron microscope(SEM). The hemocytes of Panulirus homarus are classified on the basis of whether there are granules in the cytoplasm, how many granules in it, and the size and the forms on the outside of the cells. The results show that there are three types of blood cells on the stained smears: agranular hemocyte, small granular hemocyte and large granular hemocyte. The size of them are 8.65μm×8.08μm, 12.80μm×11.30μm and (15.90)μm×13.50μm respectively. The nucleus-cytoplasm ratio is 4.88, 2.03 and 0.89 respectively. There are many shapes of hemocyte such as sphere, oval and ellipse. According to the morphology characters, a granular hemocyte, small granular hemocyte and large granular hemocyte are not the blood cells during the different growth periods in the sequence development process of hemocyte, but separate development. The counts of different hemocytes are performed by light microscope. The percentage of agranular hemocyte, small hemocyte and large hemocyte is 57.1%,18.0%,24.9% respectively. There are different percentages of the three types of hemocytes in circulation hemocyte of various crustaceans respectively. The differences relate close with the various species, the different physiological capabilities, the different growth and development periods of crustaceans. The pigmentation of three types of hemocytes shows analogy with human white cells by Wright and Geimsa. By the scanning electron microscope observation, all types of hemocytes are not smooth on the surface, where there are a lot of protuberances. It is analogous with the hemocyte and granular hemocyte of humanity. This suggests that the cell structure changes actively and implies their functions with phagocytosis and encapsulation. The granular hemocyte of Penaeus chinensis and other crustaceans have the functions of immune defense. Thus, agranular hemocyte, small granular hemocyte and large granular hemocyte of Panulirus homarus are similar to humanity's white cells and the other crustaceans in immune mechanism.