详细信息
Polysaccharide from Sepia esculenta ink and cisplatin inhibit synergistically proliferation and metastasis of triple-negative breast cancer MDA-MB-231 cells ( SCI-EXPANDED收录) 被引量:10
文献类型:期刊文献
英文题名:Polysaccharide from Sepia esculenta ink and cisplatin inhibit synergistically proliferation and metastasis of triple-negative breast cancer MDA-MB-231 cells
作者:Liu, Hua-Zhong[1];Xiao, Wei[2,3];Gu, Yi-Peng[4];Tao, Ye-Xing[5];Zhang, Da-Yan[3];Du, Hui[6];Shang, Jiang-Hua[2]
机构:[1]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Agr Sci, Guangxi Buffalo Res Inst, Nanning 530001, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[4]Hezhou Univ, Inst Food Sci & Engn Technol, Hezhou 542899, Peoples R China;[5]Guilin Med Univ, Expt Ctr Sci, Guilin 541004, Peoples R China;[6]Guangdong Ocean Univ, Biochem Ctr, Zhanjiang 524088, Peoples R China
年份:2016
卷号:19
期号:12
起止页码:1292
外文期刊名:IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
收录:SCI-EXPANDED(收录号:WOS:000391090200005)、、WOS
基金:This work was supported by the Natural Science Foundation of Guangdong Province, China (2016A030313753), the National Natural Science Foundation of China (31171667), and the Opening Project of Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding (SNKF-2014-01). The results described in this paper were part of student thesis.
语种:英文
外文关键词:Chou-Talalay method; Cisplatin; MDA-MB-231 cells; Sepia esculenta ink
外文摘要:Objective(s): This paper aims to investigate synergistic inhibition of polysaccharide from Sepia esculenta ink (SIP), a newly isolated marine polysaccharide in our laboratory, on breast cancer MDA-MB- 231 cells exposed to cisplatin. Materials and Methods: Cell viability of MDA-MB-231 cells was determined by CCK 8 assay. Median-effect concentration was analyzed using Chou-Talalay method that was also subjected to determine cell inhibition ratio and combined index, as well as interaction between SIP and cisplatin. Proliferation and migration abilities were detected with plate colony formation assay and cell wound scratch assay, respectively. Expression of MMP-2 and MMP-9 proteins was measured with Western blot assay. Results: Data showed that SIP not only suppressed proliferation and migration of MDA-MB-231 cells, and expression of MMP-2 and MMP-9 proteins, also promoted inhibition of cisplatin on proliferation, migration and MMPs expression of MDA-MB-231 cells, which indicates synergy inhibition of drug combination of SIP and cisplatin on breast cancer cells. The median-effect concentrations of cisplatin and SIP were 4.9 and 1659.6 mu g/ml, respectively. Whereas the concentration of combination drug was 158.5 mu g/ml. The data indicated that drug combination can decrease dosages of the two single agents, especially the usual dosage of cisplatin. Conclusion: This research demonstrated that SIP repressed proliferation and metastasis of MDA-MB-231 cells and promoted anticancer effect of cisplatin on the breast cancer cells. The data suggested that SIP is a potential natural drug that can be used as an auxiliary medicine alongside chemotherapy in treating breast cancer.
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