文献类型：期刊文献

英文题名：Mitochondrial damage are involved in Aflatoxin B₁-induced testicular damage and spermatogenesis disorder in mice

作者：Huang, Wanyue[1];Cao, Zheng[1];Yao, Qiucheng[2];Ji, Qiang[1];Zhang, Jian[1];Li, Yanfei[1]

机构：[1]Northeast Agr Univ, Coll Vet Med, Dept Heilongjiang Common Anim Dis Prevent & Treat, Key Lab Prov Educ, Harbin 150030, Heilongjiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524000, Peoples R China

年份：2020

卷号：701

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：SCI-EXPANDED(收录号：WOS:000498801400041)、、EI(收录号：20194607682825)、Scopus(收录号：2-s2.0-85074749625)、WOS

基金：This study was supported by the "Young Talent" Project of Northeast Agricultural University (18QC44); and the National Youth Foundation of China (31902332).

语种：英文

外文关键词：Aflatoxin B-1 (AFB(1)); Mitochondria; Testicular damage; Spermatogenesis disorder; Mice

外文摘要：Aflatoxin B-1 (AFB(1)) is an unavoidable environmental pollutants, which seriously endangers human and animal health. AFB(1) has male reproductive toxicity, yet the underlying mechanisms remain inconclusive. Mitochondra are a kind of crucial organelle for maintaining spermatogenesis in testis. Thus, we hypothesized that AFB(1) can impair mitochondria to aggravate testicular damage and spermatogenesis disorder. To verify this hypothesis, 48 male mice were intragastrically administered with 0, 0.375, 0.75 or 1.5 mg/kg body weight AFB(1) for 30 days, respectively. In this study, we found AFB1 caused testicular histopathological lesions and spermatogenesis abnormalities, with the elevation of oxidative stress (increased H2O2, whereas decreased SOD and GSH). Significant mitochondria structure damage of germ cells and Leydig cells, MMP loss, ATP contents reduction, and inhibited activities of mitochondrial complexes I-IV in mice testis were found in AFB(1) treatment groups. Besides, AFB1 inhibited mitochondrial biogenesis and mitochondrial dynamics, presenting as the decreased mRNA and protein expressions of PGC-1 alpha, Nrf1, Tfam, Drp1, Fis1, Mfn1 and Opa1. The results revealed that the mitochondrial damage were involved in AFB(1)-induced testicular damage and spermatogenesis disorder, providing a considerable direction to clarify potential mechanisms of AFB(1) reproductive toxicity. (C) 2019 Elsevier B.V. All rights reserved.