文献类型：期刊文献

英文题名：The physiological responses to titanium dioxide nanoparticles exposure in pearl oysters (Pinctada fucata martensii)

作者：Li, Fengfeng[1];Xie, Yufen[1];Yang, Chuangye[1,2,3,4];Ye, Qingxia[1];Wang, Feiyu[1];Liao, Yongshan[5];Mkuye, Robert[1];Deng, Yuewen[1,2,3,4,6]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Aquatic Anim Dis Control &, Zhanjiang 524088, Peoples R China;[3]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[4]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China;[5]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang, Peoples R China;[6]Guangdong Marine Ecol Early Warning & Monitoring L, Zhanjiang 524088, Peoples R China

年份：2024

卷号：195

外文期刊名：MARINE ENVIRONMENTAL RESEARCH

收录：SCI-EXPANDED(收录号：WOS:001165541000001)、、EI(收录号：20240315399340)、Scopus(收录号：2-s2.0-85182507551)、WOS

基金：This work was supported by the program for scientific research start- up funds of Guangdong Ocean University (Grant No. 060302022304) , National Natural Science Foundation of China (Grant No. 32102817) , Students Innovation and Entrepreneurship Training Program of Guangdong Ocean University (Grant No. CXXL2023003) , Department of Education of Guangdong Province (Grant No. 2020ZDZX1045 and 2021KCXTD026) , the earmarked fund for CARS -49, and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (Grant No. 2023KJ146) . We are very grateful to Marine Pearl Science and Technology Backyard in Leizhou of Guangdong for collecting samples. The authors would like to thank TopEdit ( www.topeditsci.com ) for its linguistic assistance during the preparation.

语种：英文

外文关键词：TiO2 nanoparticles; Digestion; Oxidative stress; Biomineralization; Pinctada fucata martensii

外文摘要：To evaluate the physiological responses to titanium dioxide nanoparticles exposure in pearl oysters (Pinctada fucata martensii), pearl oysters were exposed for 14 days to different levels (0.05, 0.5, and 5 mg/L) of nano-TiO2 suspensions, while a control group did not undergo any nano-TiO2 treatment. And then recovery experiments were performed for 7 days without nano-TiO2 exposure. At days 1, 3, 7, 14, 17, and 21, hepatopancreatic tissue samples were collected and used to examine the activities of protease, amylase, lipase, catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), lysozyme (LYS), alkaline phosphatase (AKP), and acid phosphatase (ACP). The microstructure of the nacreous layer in shell was also analyzed by scanning electron microscopy. Results showed that pearl oysters exposed to 5 mg/L of TiO2 nanoparticles had significantly lower protease, amylase, and lipase activities and significantly higher CAT, SOD, GPx, LYS, ACP, and AKP activities than control pearl oysters did even after 7-day recovery (P-values <0.05). Pearl oysters exposed to 0.5 mg/L or 0.05 mg/L of TiO2 nanoparticles had lower protease, amylase, and lipase activities and higher CAT, SOD, GPx, LYS, ACP, and AKP activities than control pearl oysters did during the exposure period. After 7-day recovery, no significant differences in protease, lipase, SOD, GPx, CAT, ACP, AKP, or LYS activities were observed between pearl oysters exposed to 0.05 mg/L of TiO2 nanoparticles and control pearl oysters (P-values >0.05). In the period from day 7 to day 14, indistinct and irregular nacreous layer crystal structure in shell was observed. This study demonstrates that TiO2 nanoparticles exposure influences the levels of digestion, immune function, oxidative stress, and biomineralization in pearl oysters, which can be partially and weakly alleviated by short-term recovery. These findings contribute to understanding the mechanisms of action of TiO2 nanoparticles in bivalves. However, studies should evaluate whether a longer recovery period can restore to their normal levels in the future.