文献类型：期刊文献

英文题名：Development of SSR marker by RNA-seq and its application in genotyping pearl sac in pearl oyster Pinctada fucata martensii

作者：Lei, Chao[1];Bei, Weilie[1];Wang, Xueying[1];Du, Xiaodong[1];Jiao, Yu[1];Huang, Ronglian[1];Wang, Qingheng[1];Deng, Yuewen[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524025, Guangdong, Peoples R China

年份：2017

卷号：25

起止页码：70

外文期刊名：ELECTRONIC JOURNAL OF BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000392435900012)、、EI(收录号：20170303262724)、Scopus(收录号：2-s2.0-85009438161)、WOS

基金：The study was financially supported by the National Natural Science Foundation of China (31372526), the Administration of Ocean and Fisheries of Guangdong Province (Z2014011 and A201308A10) and "Haizhifan-qihang" Project of Guangdong Ocean University.

语种：英文

外文关键词：Pinctada fucata martensii; SSR development; Pearl sac; Donor oyster; Host oyster; Genotyping

外文摘要：Background: Pearl oyster Pinctada fucata martensii is cultured for producing round nucleated pearls. Pearl production involves a surgical operation where a mantle tissue graft from a donor oyster and a round nucleus are implanted in the gonad of a host oyster. Whether the mantle graft implanted in the gonad of a host oyster contributes to the formation of a pearl sac that secretes pearl nacre to form a pearl should be determined. In April 2012, two full-sib families were separately used as donor and host oysters for a nucleus insertion operation. The pearl sac was sampled from the host oysters at day 60 after nucleus operation. A large number of simple sequence repeat (SSR) markers were developed using Illumina HiSeq (TM) 2000 platform. The two full-sib families were also used to mine diagnostic SSR markers for genotyping donor oyster, host oyster, and pearl sac. Results: A total of 3168 microsatellite loci were identified in 39,078 unigenes, and 1977 SSR primers were designed by Primer 3.0. Forty-seven SSR primers were validated, and the rate of successful amplification was 72.3%. Two diagnostic SSR primers could successfully genotype pearl sac, donor oyster, and host oyster. Donor and host oysters were both homogenous, and the alleles in pearl sac were identical to those in donor and host oysters. Conclusions: The present results confirmed that the mantle graft implanted in the gonad of host oyster contributed to the formation of the pearl sac in pearl oyster P. fucata martensii. (C) 2016 PontificiaUniversidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved. This is an open access article under the CC BY-NC-ND license